内毒素血症大鼠肝损伤及中药912液干预的研究

目的探讨内毒素血症模型大鼠肝损伤的情况以及中药912液的保护作用。方法将大鼠随机分模型组、干预组及正常对照组,模型组及干预组采用脂多糖（lipoplysaccharides,LPS）腹腔注射造模。干预组在制模前1h给予中药912液灌胃,于2h、4h、8h、12h、24h、48h、72h及7天取血,检查肝功能、血浆内毒素、TNF-α、IL-10等指标;取肝组织,光镜及透射电镜观察。结果干预组血浆内毒素（Eu/mL）水平2h最高（0.358±0.056）,显著低于模型组2h水平（0.685±0.03）;两组血清TNF-α（ng/L）水平升高差异无显著性（P〉0.05）。中药912液干预组的IL-10（ng/L）水平一直持续升高,第7天（49.096±4.076）时仍高于正常对照组（43.454±5.928）,差异有显著性（P〈0.05）。结论LPS可以导致大鼠血清中致炎因子和抗炎因子增加,肝功能损伤,推测LPS所致的机体炎症反应可能是肝损伤的机理之一。中药912液预防给药后对肝脏有保护作用。

Liver Injury and Intervention of Compound 912 Liquid on It in Rats with Endotoxemia

OBJECTIVE: To investigate the liver injury in model rats with endotoxemia and to observe the protective effect of Compound 912 Liquid on it. METHODS: Rats were randomly divided into three groups, the endotoxemia model group (EMG, injected by lipoplysaccharides (LPS) peritoneally), the intervention group (IG, treated with Compound 912 Liquid via gastrogavage 1 h before model establishing) and the normal control group (NCG). Blood samples of rats were taken at the time points of the 2nd, 4th, 8th, 12th, 48th, 72nd hour and the 7th day after modeling for measuring liver function, levels of plasmatic endotoxin, tumor necrosis factor alpha (TNF-alpha), interleukin-10 (IL-10). The pathological change of liver was observed using light microscope and electro-transmission microscope. RESULTS: The peak concentration of endotoxin detected at 2 hour after modeling in the IG was significantly lower than that in the EMG (0.358 +/- 0.056 vs 0.685 +/- 0.030), but insignificant difference (P > 0.05) was shown between them in TNF-alpha level. The level of IL-10 continuously rose in IG after treatment, it was still higher than normal level until day 7 (49.096 +/- 4.076 vs 43.454 +/- 5.928, P < 0.05). CONCLUSION: LPS can induce the increase of serum inflammatory cytokines and anti-inflammatory cytokines in rats to injure liver. Therefore, the inflammatory reaction indicated by LPS may be one of the mechanisms for liver injury. Preventive medication with Compound 912 Liquid showed a significant liver protective effect.