| 实时定量PCR检测正常人外周血T细胞和胸腺细胞中sjTRECs水平 |
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| 引用本文： | 李扬秋,杨力建,陈少华,陈嘉愉,刘薇薇,王震,张玉平,吴秀丽.实时定量PCR检测正常人外周血T细胞和胸腺细胞中sjTRECs水平[J].现代临床医学生物工程学杂志,2001,7(6):397-400. |
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| 作者姓名： | 李扬秋 杨力建 陈少华 陈嘉愉 刘薇薇 王震 张玉平 吴秀丽 |
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| 作者单位： | 李扬秋（暨南大学医学院血液病研究所,广州,510632）
杨力建（暨南大学医学院血液病研究所,广州,510632）
陈少华（暨南大学医学院血液病研究所,广州,510632）
陈嘉愉（广州市第十二人民医院,广州,510620）
刘薇薇（广州市第十二人民医院,广州,510620）
王震（暨南大学医学院血液病研究所,广州,510632）
张玉平（暨南大学医学院血液病研究所,广州,510632）
吴秀丽（暨南大学医学院血液病研究所,广州,510632） |
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| 基金项目： | 国家教育部科技重点项目(编号:教技司[2001]235号01091)和广州市科技攻关项目 (编号:2001-Z-037-01-3) |
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| 摘 要： | 目的了解正常人外周血T细胞和胸腺细胞中信号结合T细胞受体删除 DNA环(sjTRECs)的含量,从而推测正常人中幼稚T细胞的含量和胸腺的输出功能. 方法利用实时定量PCR和TaqMan方法检测11例正常人外周血单个核细胞、7例儿童和3例成人胸腺细胞DNA中sjTRECs的水平. 结果正常人中sjTRECs含量分别为8.83±4.81/1000个外周血单个核细胞;27.31±3.23/1000个成人胸腺细胞和170 .29 ±59.52/1000个儿童胸腺细胞. 结论 sjTRECs水平与年龄有关,正常人外周血中每1000个单个核细胞中约含4.5个幼稚T细胞.
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| 关 键 词： | sjTRECs 幼稚T细胞 胸腺细胞 实时定量PCR |
| 文章编号： | 1008-634X(2001)06-0397-03 |
| 修稿时间： | 2001年11月15 |
Detection of sjTRECs within Peripheral Blood T Cells and Thymucytes in Normal Individuals by Real-time PCR |
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| LI Yang qiu,YANG Li jian,CHEN Shao hua,et al..Detection of sjTRECs within Peripheral Blood T Cells and Thymucytes in Normal Individuals by Real-time PCR[J].Journal of Modern Clinical Medical Bioengineering,2001,7(6):397-400. |
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| Authors: | LI Yang qiu YANG Li jian CHEN Shao hua |
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| Abstract: | Objective To analyze the content of signal joint T-cell receptor excision DNA circles(sjTRECs) within peripheral blood mononuclear cells(PBMCs) and thymucytes in normal individuals,thereby to suggest the content of naive T cells and the thymic output function in normal individuals. Methods Quantitative detection of sjTRECs in DNA of peripheral blood mononuclear cells from 11 normal individuals and thymucytes from 7 children and 3 adults were preformed by real-time PCR and TaqMan analysis. Results The mean value of sjTRECs in normal individuals is 8.83 4.81/1000 cells in PBMCs,27.31 3.23/1000 cells in adult thymus and 170.29 59.52/1000 cells in children thymus,respectively. Conclusions The results indicate that the sjTRECs levels may correlate with the age and the number of naive T cells in PBMCs was about 4.5 per 1000 cells. |
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| Keywords: | Signal joint T cell receptor excision DNA circles (sjTRECs) Naive T cells Thymucytes Real-time PCR |
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