吡格列酮对人脐静脉内皮细胞血凝素样氧化低密度脂蛋白受体1表达的影响

目的探讨氧化型低密度脂蛋白(ox-LDL)作用下,吡格列酮对人脐静脉内皮细胞(HUVECs)血凝素样氧化低密度脂蛋白受体1(LOX-1)表达的影响。方法将培养的第4代HUVECs分5组:空白组(无干预);ox-LDL组(80 mg/L ox-LDL);低浓度组(1μmol/L吡格列酮+80 mg/L ox-LDL);高浓度组(10μmol/L吡格列酮+80 mg/Lox-LDL);对照组(10μmol/L吡格列酮),各组培养24 h后,采用流式细胞仪检测LOX-1的表达,采用RT-PCR检测LOX-1 mRNA的表达。结果与空白组比较,对照组LOX-1及LOX-1 mRNA表达均无明显改变(P0.05),ox-LDL组、低浓度组和高浓度组LOX-1及LOX-1 mRNA表达明显增多(P0.01);与ox-LDL组比较,低浓度组和高浓度组LOX-1及LOX-1 mRNA表达明显降低(P0.01);高浓度组较低浓度组降低更明显(P0.05)。结论吡格列酮能降低ox-LDL刺激下的HUVECs LOX-1及LOX-1 mRNA的表达,提示吡格列酮可能通过干预ox-LDL的病理生理过程起到抗动脉硬化的作用。

The effects of pioglitazone on LOX-1 expression on human umbilical vein endothelial cells

Objective To investigate the effect of pioglitazone on lectin-like exidized LDL receptor (LOX-1) and LOX-1 mRNA expression on human umbilical vein endothelial cells(HUVECs) induced by oxidized low-density lipoprotein (ox-LDL).Methods HUVECs were divided into 5 groups:the blank group (no stimulation and intervention) ;the ox-LDL group(80 mg/L ox-LDL) ; the low concentration group(1μmol/L pioglitazone+ 80 mg/L ox-LDL) ;the high concentration group(10μmol/L pioglitazone+80 mg/L ox-LDL);the control group(10 μmol/L pioglitazone). Expression of LOX-1 was detected by flow cytometric technology,LOX-1 mRNA expression was evaluated by RT-PCR.Results Compared with the blank group,LOX-1 and LOX-1 mRNA expression was significantly increased in the ox-LDL group,the high concentration and the low concentration groups (P < 0.01).Compared with ox-LDL group,LOX-1 and LOX-1 mRNA expression was significantly decreased in the high concentration group and the low concentration group (P < 0.01) and the expression was decreased more significantly in the high concentration group.Conclusion Pioglitazone can inhibit the upregulation of LOX-1 and LOX-1 mRNA on HUVECs induced by ox-LDL.These results provide novel insight into the anti-atherosclerotic effects of pioglitazone,which may be related to its intervention on pathophysiologic effects of ox-LDL.