Survivin和Bcl-2在尖锐湿疣中的表达

目的:测定survivin和Bcl-2在尖锐湿疣(CA)组织中的表达并探讨两者表达的关系。方法:应用免疫组织化学链霉菌抗生物素蛋白过氧化物酶方法(SP法)分别检测60例CA组织及10例正常包皮组织中survivin、Bcl-2基因的表达。在镜下观察切片并计算阳性细胞百分比。结果:CA组织中survivin的表达率为86.67%,显著高于正常包皮的表达率20%(P<0.01)。其中棘层的表达率85.00%显著高于基底层的表达率70.00%和颗粒层的表达率66.67%(P<0.05),基底层与颗粒层的表达率之间无显著性差异(P>0.05)。Bcl-2在CA的表达率为66.67%,正常包皮组织中未见Bcl-2蛋白表达。survivin在CA中的表达与Bcl-2呈正相关(P<0.01)。结论:本实验检测出survivin基因在CA组织中有异常表达,提示survivin蛋白引起的细胞凋亡抑制在CA的发生中起到重要的作用。survivin与Bcl-2在CA的发生发展过程中起到协同作用。

The expression of survivin and Bcl- 2 in condyloma acuminatum

Objective:To measure the expression of survivin and Bcl-2 and to determine the relationship between the expressions of survivin and Bcl-2 in condyloma acuminatum(CA).Method:Immunohistochemical SP method was employed to measure the expressions of survivin and Bcl-2 in 60 cases of CA and specimens of prepuce of 10 male adults.The percentage of positive cells was calculated under microscope.Results:Survivin was expressed in 52(86.67%)CA cases and 2(20%)prepuce tissues.The expression of survivin was significantly higher in CA than that in prepuce tissues(P<0.01).The positivity rate in stratum spinosum was 85%,which was higher than 70% in basal layer(70%)and 66.67% in stratum granulosum(all P<0.05).The difference in the expressions between basal layer and stratum granulosum was not significant.The positivity rate of Bcl-2 in CA was 66.67%,while no expression of Bcl-2 in prepuce tissues.Expression of survivin was positively correlated with Bcl-2(P<0.01).Conclusions:Survivin may play an important role in the pathway of development of CA.Bcl-2 may have a synergic role with survivin in the development of CA.