免疫抑制剂对口腔白色念珠菌的分布及基因分型的影响

目的:研究服用免疫抑制剂患者口腔中白色念珠菌的分布及基因分型。方法:含漱液浓缩法取样,CHROM agar Cand ida鉴定培养基分离鉴定,PCR方法鉴定白色念珠菌和基因分型,PCR结果测序验证。结果:实验组白色念珠菌的检出率为20%,对照组7.69%,差异有显著性,实验组中多种念珠菌共存的现象多见。对照组白色念珠菌基因型以A型为主,实验组白色念珠菌基因型呈多态性。结论:长期服用免疫抑制剂影响口腔念珠菌及不同基因型白色念珠菌的检出。

Incidence and genotype of oral Candida albican from drug-immuno-suppressed patients

Objective:To find the influence of the immuno-suppressive drug on the incidence and genotype of oral Candida albicans.Methods:51 patients using immuno-suppressive drug for 1 to 3 months were enrolled,the controls were 26 health subjects.Oral rinse samples from the patients and the controls were assessed for the growth of yeast.The liquid samples were plated on CHROMagar Candida.The isolates were defined to the species level and the genotypic subgroups by PCR method.PCR results were proved by sequencing.Results:The incidence of Candida albican in the patients and controls was 20% and 7.69% respectively.The genotype of the Candida albican in the controls is A subgroup,that in the patients included A,B and C subgroups.Conclusion:Long term of immuno-suppressive drug use can influence the genotype and incidence of oral Candida albican.