MMP-9/TIMP-1与肝癌侵袭转移及病毒抗原表达的相关性研究

目的:探讨基质金属蛋白酶-9(MMP-9)及其抑制剂-1(TIMP-1)在人肝癌及癌旁组织中的表达和肝癌生物学行为及病毒抗原表达之间的关系。方法:应用免疫组织化学法(SP)研究40例肝癌及癌旁组织中MMP-9、TIMP-1和HBsAg、HCV抗原的表达。结果:MMP-9在肝癌和癌旁组织表达阳性率分别为70.25%和75%,癌组织明显减低(P<0.01)。TIMP-1在肝癌组织和癌旁组织中的表达阳性率分别为57.5%和62.5%,肝癌中的表达较癌旁肝组织表达减低,但差异无显著性。转移组癌及癌旁组织MMP-9的表达显著高于无转移组(P<0.05);而TIMP-1则明显低于无转移组(P<0.05)。肝癌、癌旁组织MMP-9、TIMP-1表达随着无病毒感染、单一病毒感染、双重感染分组而增高,且MMP-9表达与病毒抗原表达明显相关(P<0.05)。HBsAg、HCV虽可在癌组织及癌旁组织表达,但有一定差异:HBsAg主要在癌旁组织表达(70%),高于癌组织表达(27.5%);而HCV在癌组织表达(27.5%)与癌旁组织表达(30%)水平相当。结论:MMP-9、TIMP-1在肝癌及癌旁组织中的表达明显增高,可能对于判断肝癌的侵润、转移有重要价值;HBV、HCV感染可能与MMP-9表达增加有关。

Study of the expression of MMP-9/TIMP-1 and the relationship between MMP-9/TIMP-1 and HBsAg,HCV expression in cancerous and pericancer tissues of hepatocellular carcinomar

Objective:To study the expression of MMP-9/TIMP-1 in cancerous and pericancer tissues of hepatocellular carcinomar and the relationshi between their expression and liver cancer biology behavior,HBsAg,HCV.Methods:The patterns of MMP-9,TIMP-1 and HBsAg,HCV in 40 cases of HCC and their surrounding liver tissues were studied with paraffin sections using immunohistochemistry techniques.Results:The postive rate of MMP-9 protein was 70.25% in HCC,and was markedly decreased than in paratumor samples 75%(P<0.01).The positive rate of TIMP-1 protein in carcinomar and the tissue close to tumor was 57.5% and 62.5%,there is no difference between them(P<0.05).In the transfered group the expression of MMP-9 is much higher and the expression of TIMP-1 is markedly lower than in non-transfered in both cancerous and pericancer tissues of HCC(P<0.05).The expression of MMP-9 and TIMP-1 was guadually increasing from non-infection of HBV,HCV group to pure HBV or HCV infected group to co-infection group in both cancerous and pericancer tissues of HCC.HBV,HCV virus infection were positively correlated wit MMP-9(P<0.05).HBsAg and HCV were detected both in HCC and pericarcinomatous tissues.The positive rate of HBsAg in pericarcinomatous tissues(70%) was higher than that of HCC,tissues(27.5%);HCV expression in HCC(27.5%) and pericarcinomatous tissues(70%) had no differences.Conclusions:MMP-9 and TIMP-1 is over-expressed in both cancerous and pericancer tissues of HCC,and they may have importmant value in determining whether there are metastasis or transference;HBV,HCV infection may increase tehe expression of MMP-9.