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被动免疫预防犊牛轮状病毒感染的研究
引用本文:张俭伟,杨少华,王长法,高运东,杨宏军,仲跻峰,赵宏坤. 被动免疫预防犊牛轮状病毒感染的研究[J]. 中国人兽共患病杂志, 2007, 23(6): 569-571,579
作者姓名:张俭伟  杨少华  王长法  高运东  杨宏军  仲跻峰  赵宏坤
作者单位:[1]山东农业大学动物科技学院,泰安271018 [2]山东省农业科学院奶牛研究中心,泰安271018
基金项目:山东省农业良种产业化工程项目;国家科技攻关项目
摘    要:目的 轮状病毒灭活疫苗免疫孕牛后,研究产下犊牛通过母源抗体获得的被动免疫在预防牛轮状病毒感染中的作用。方法 孕牛产前2月和1月分别接种轮状病毒灭活疫苗,检测产牛后乳汁和犊牛血样中BRV特异性IgG、IgA及中和抗体滴度。犊牛21日龄时口服2mL10^4,59 TCID50/ml的牛轮状病毒,观察犊牛腹泻及排毒情况。结果 免疫组乳清和犊牛血清中产生了高水平的牛轮状病毒特异性IgG、IgA及中和抗体,与对照组差异极显著(P〈0.01);攻毒后,对照组6头犊牛全部发生腹泻,免疫组6头中有1头腹泻,发病的潜伏期及发病率差异显著。结论 被动免疫可显著提高犊牛血清中牛轮状病毒特异性抗体水平,使犊牛获得一定的免疫保护力抵抗轮状病毒的感染。

关 键 词:产前免疫  牛轮状病毒  灭活疫苗
文章编号:1002-2694(2007)06-0569-03
修稿时间:2006-11-152007-01-04

Passive antibody immunization against bovine rotavirus infection
ZHANG Jian-wei,YANG Shao-hua,WANG Chang-fa,GAO Yun-dong,YANG Hong-jun,ZHONG Ji-feng,ZHAO Hong-kun. Passive antibody immunization against bovine rotavirus infection[J]. Chinese Journal of Zoonoses, 2007, 23(6): 569-571,579
Authors:ZHANG Jian-wei  YANG Shao-hua  WANG Chang-fa  GAO Yun-dong  YANG Hong-jun  ZHONG Ji-feng  ZHAO Hong-kun
Affiliation:College of Veterinary Medicine, Shandong Agricultural University, Taij an 271018, China
Abstract:Cows were immunized with bovine rotavirus vaccine and then the effect of passive antibody from colostrum and serum on the immunity against bovine rotavirus infection was investigated, in which cows were immunized at two and one month before calving, and IgG, IgA and neutralizing rotavirus antibody in colostrum or serum were determined. A virulent bovine rotavirus was given orally to the calves at 21 days after birth, and the infection rate after challenge were compared. It was demonstrated that the titer of the anti-rotavirus antibody in colostrum and serum in the vaccinated group of cows was significantly increased compared with that of the control group (P<0.01), however, 6 calves born from control cows and one calve born from vaccinated cows developed diarrhea after challenge. The incubation periods and the infection rates appeared to be quite different between these two groups of cows(P<0.01). It is apparent that the passive antibody immunization can elevate significantly the level of the specific antibodies against bovine rotavirus infection and can prevent infection after rotavirus challenge.
Keywords:passive immunity   bovine rotavirus   infected vaccine
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