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No donor age effect of human serum on collagen synthesis signaling and cell proliferation of human tendon fibroblasts |
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| Authors: | Bayer Monika L Schjerling Peter Biskup Edyta Herchenhan Andreas Heinemeier Katja M Doessing Simon Krogsgaard Michael Kjaer Michael |
| Affiliation: | a Institute of Sports Medicine, Department of Orthopedic Surgery M, Bispebjerg Hospital and Center for Healthy Aging, Faculty of Health Sciences, University of Copenhagen, Denmark b Department of Dermatology, Bispebjerg Hospital, University of Copenhagen, Denmark c Department of Sports Traumatology and Arthroscopy, Bispebjerg Hospital, University of Copenhagen, Denmark |
| Abstract: | The aging process of tendon tissue is associated with decreased collagen content and increased risk for injuries. An essential factor in tendon physiology is transforming growth factor-β1 (TGF-β1), which is presumed to be reduced systemically with advanced age. The aim of this study was to investigate whether human serum from elderly donors would have an inhibiting effect on the expression of collagen and collagen-related genes as well as on cell proliferative capacity in tendon cells from young individuals. There was no difference in systemic TGF-β1 levels in serum obtained from young and elderly donors, and we found no difference in collagen expression when cells were subjected to human serum from elderly versus young donors. In addition, tendon cell proliferation was similar when culture medium was supplemented with serum of different donor age. These findings suggest that factors such as the cell intrinsic capacity or the tissue-specific environment rather than systemic circulating factors are important for functional capacity throughout life in human tendon cells. |
| Keywords: | Extracellular matrix Tendon Humoral factors Collagen Cell proliferation |
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