| 三氧化二砷和四乙铵对人肺腺癌细胞株AGZY-83-a增殖和凋亡影响的研究 |
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| 引用本文： | 李伟,陈英准,丁秀娟,李雪连,薛小荣,单宏丽. 三氧化二砷和四乙铵对人肺腺癌细胞株AGZY-83-a增殖和凋亡影响的研究[J]. 哈尔滨医科大学学报, 2010, 44(1) |
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| 作者姓名： | 李伟 陈英准 丁秀娟 李雪连 薛小荣 单宏丽 |
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| 作者单位： | 哈尔滨医科大学,药理学教研室,省部共建国家重点实验室培育基地;哈尔滨医科大学第二临床医学院,病理科,黑龙江,哈尔滨,150081;黑龙江省齐齐哈尔市第一医院,心内四科,黑龙江,齐齐哈尔,161005 |
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| 基金项目： | 黑龙江省卫生厅项目(2006-328);;黑龙江省教育厅项目(11531131) |
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| 摘 要： | 目的研究三氧化二砷(arsenic trioxide,As2O3)和四乙铵(tetraethylammonium,TEA)对人肺腺癌细胞AG-ZY-83-a增殖和凋亡的影响,探讨As2O3诱导人肺腺癌细胞株凋亡的机制。方法体外细胞培养人肺腺癌AGZY-83-a细胞株,采用MTT法检测As2O3和TEA对细胞增殖的影响。透射电镜(TEM)、流式细胞术(FCM)检测细胞凋亡情况。比色法检测细胞内Caspase-3活性改变。结果As2O3和TEA对AGZY-83-a细胞增殖有明显的抑制作用(P<0.01)。As2O3组对AGZY-83-a细胞增殖抑制率低于TEA+As2O3组(P<0.05)。As2O3和TEA使AGZY-83-a细胞凋亡率,Caspase-3增加,单用As2O3组的细胞凋亡率、Caspase-3活性分别高于TEA+As2O3组(P<0.05)。结论应用TEA部分抑制了As2O3诱导AGZY-83-a细胞凋亡;As2O3诱导AGZY-83-a细胞凋亡可能和As2O3对细胞膜不同的钾离子通道作用有关系。
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| 关 键 词： | 三氧化二砷 四乙铵 AGZY-83-a 凋亡 半胱氨酸酶-3 |
Effect of arsenic trioxide and tetraethylammonium on proliferation and apoptosis of human pulmonary adenocarcinoma AGZY-83-a cells |
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| LI Wei,CHEN Ying-zhun,DING Xiu-juan,et al. Effect of arsenic trioxide and tetraethylammonium on proliferation and apoptosis of human pulmonary adenocarcinoma AGZY-83-a cells[J]. Journal of Harbin Medical University, 2010, 44(1) |
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| Authors: | LI Wei CHEN Ying-zhun DING Xiu-juan et al |
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| Affiliation: | Department of Pharmacology;Bio-pharmaceutical Key Laboratory of HeilongjiangProvince-Incubator of State Key Laboratory;Harbin 150081;China |
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| Abstract: | Objective To investigate the effect of arsenic trioxide(As2O3) and tetraethylammonium on proliferation and apoptosis of human pulmonary adenocarcinoma AGZY-83-a cell line and its mechanism.Methods The inhibitory rate of cultured AGZY-83-a cells treated with As2O3,TEA was observed by MTT assay.Apoptosis was detected by flow cytometry and electronic microscope.Cellular caspase-3 activities were observed with colorimetric method.Results The proliferation of AGZY-83-a cells was inhibited by As2O3 and TEA(P<0.01... |
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| Keywords: | arsenic trioxide TEA AGZY-83-a apoptosis Caspase-3 |
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