苏黄止咳颗粒平喘止咳作用的实验研究

目的:观察苏黄止咳颗粒平喘、止咳、祛痰作用。方法:采用乙酰胆碱与组织胺等量混合液引喘法、致敏动物抗原攻击法、豚鼠气管段法观察其平喘作用;采用氨水、枸橼酸喷雾致咳法观察其止咳作用;采用气管段酚红排出法观察其祛痰作用。结果:苏黄止咳颗粒可显著延长乙酰胆碱与组织胺混合液引发豚鼠抽搐的潜伏期,降低致敏动物抗原攻击致哮喘豚鼠气管肺灌流液中白细胞数及腹腔液中肥大细胞脱颗粒发生率,明显降低气管平滑肌张力;显著延长枸橼酸喷雾引咳豚鼠的咳嗽潜伏期,减少其咳嗽次数,延长氨水喷雾致咳小鼠的半数小鼠咳嗽时间;并增加小鼠气管对酚红的排出量。结论:苏黄止咳颗粒具有明显平喘、止咳、祛痰作用。

Studies on Antitussive and Antiasthmatic Action of Suhuangzhike Granules

Objective:To study the antiasthmatic,antitussive,and expectorant effects of Suhuangzhike granules.Method:Using the asthma model induced by acetylcholine and histamine mixture,antigen challenging(ovalbumin)and guinea pig' isolated trachea strip test the antiasthmatic effect was evaluated;using the cough model induced by ammonia,citric acid in mice,the antitussive effect was studied;phenol red test was used to observe the expectorant effect in mice.Result:Suhuangzhike granules could significantly prolong the latent period of twitch of guinea pig caused by acetylcholine and histamine,markedly decrease the number of leucocyte in perfusate of trachea and lung;and the degranulation rate of labrocyte in peritoneal fluid of guinea pig challenged by antigen,significantly decrease the tension of smooth muscle of trachea,significantly prolong the latent period of cough and decrease the frequency of cough induced by citric acid in guinea pig,significantly inhibit the cough induced by ammonia in mice,and increase phenol red excretion of trachea quantity in mice.Conclusion:Suhuangzhike granules has antitussive,antiasthmatic and expectorant actions.