MDR相关性单克隆抗体MGr－1逆转胃癌细胞耐药的体外研究

目的：研究胃癌细胞耐药相关性单克隆抗体ＭＧｒ－１对耐长春新碱胃癌细胞（ＳＧＣ７９０１／ＶＣＲ）多药耐药性（ＭＤＲ）的影响。方法：用ＭＴＴ法检测阿霉素的细胞毒性；用流式细胞仪检测阿霉素在细胞内的蓄积及潴留。结果：ＭＧｒ－１能明显逆转ＳＧＣ７９０１／ＶＣＲ细胞对阿霉素的耐药性（Ｐ＜０．０１），并可增加ＳＧＣ７９０１／ＶＣＲ细胞内阿霉素的蓄积（Ｐ＜０．０１）及潴留（Ｐ＜０．０１）。结论：ＭＧｒ－１具有通过增加细胞内药物蓄积及潴留逆转ＳＧＣ７９０１／ＶＣＲ细胞耐药性的功能。提示ＭＧｒ－１所对应的细胞膜抗原可能为一种新的有活性功能并与ＭＤＲ相关的分子。

An in Vitro Study on Reverse Activity of MDR Associated Monoclonal Antibody MGr-1 on Multidrug Resistance of Gastric Cancer Cell Line

Objective:Tostudy the effect of human gastric cancer cell multidrug resistance-associated monoclonal antibody on multidrug-resistance(MDR) in Vincristine-resistance human gastric cancer cell line (SGC7901/VCR). Methods: The drug sensitivity of SGC7901 and SGC7901/VCR was determined by the MTT assay. Intracellular accumulation and retention of adriamycin were estimated by flow cytometric analysis. Results: MGr-1 significantly increased the drug sensitivity of SGC7901/VCR (P<0. 01). Intracellular accumulation and retention of adriamycin were significantly increased by MGr-1 in SGC7901/VCR(P<0. 001). Conclusion: MGr-l possesses function of reversing MDR by increasing intracellular adriamycin accumulation and retention, suggesting that MGr-1 Ag may respresent another mechanism responsible for MDR in VCR-resistant human gastric cancer cell line (SGC7901/VCR).