涎腺良性多形性腺瘤和恶性多形性腺瘤中C-H-ras和P53蛋白产物的表达及其意义

目的:探讨癌基因在涎腺良恶性多形性腺瘤中的过度表达及其意义。方法:采用免疫组织化学方法对5例瘤旁腺体,21例良性多形性腺瘤和24例恶性多形性腺瘤中C-H-ras和P53蛋白产物的表达进行形态学观察。结果:5例瘤旁腺体导管上皮细胞浆C-H-ras呈阳性表达,而P53未见表达。C-H-ras在良恶性多形性腺瘤中均有过度表达,表达率分别为48％和38％。良性多形性腺瘤中主要分布于腺管样结构及部分实性条索或团块的腺上皮细胞的胞浆中。在恶性多形性肿瘤中主要分布于腺癌细胞的胞浆;P53在良恶性多形性腺瘤中也均见过度表达,表达率分别为19％和50％。P53蛋白有核型及核/浆型两种表达形式。良性多形性腺瘤中以核/浆型为主,主要分布于腺管样结构的腺上皮细胞的胞浆中。恶性多形性腺瘤中以核型为主,主要分布于腺癌细胞的细胞核。结论:C-H-ras蛋白产物的过度表达在涎腺多形性腺瘤的发生中起着重要作用,而P53在涎腺多形性腺瘤的癌变及癌进展中起着重要作用。它们共同作用促进了涎腺多形性腺瘤的发生。

Expression of C-H-ras and P53 oncoprotein in benign and malignant pleomorphk adenomas of salivary gland

Objective : To investigate expression of oncogene and its significance in benign and malignant preomorphic adenomas of salivary gland. Method: Using immunohistochemically method, expression of C -H - ras and p53 oncoprotein were detected in 21 cases of benign and 24 cases of malignant pleomorphic adenoma? of salivany gland. Results: Salivary gland ducta! epithelial cells near tumors were positive for C -H -ras, but p53 not positive. C -H -ras were overexpressed in benign and inalignent pleomorphic adenomas. The positive rates were 48% and 38% respectively. The positive staining mainly rested on the cytoplasms of grandular epithelial cells in the tubular structures in benign pleomorphic adenomas, but on cytoplasms of adenocarcinoma cells in malignant pleomorphic adenomas; p53 were also overexpressed in benign and malignent pleomorphic adenomas. The positive rates were 19% and 50% respectively. Expression of p53 have neclei and neclei/cytoplasms types. In benign pleomorphic adenomas, the positive staining mainly distributed on neclei/cytoplasms of grandular epithelial cells in the tubular structures,But on neclei of adenocarcinoma cells in malignant pleomorphic adenomas. Conclusion: Overexpression of C -H -ras nocoprotein played an important role in the histogenesis of salivary gland tumors, p53 nocoprotein played an important role in the carcinogenesis and development of salivary gland tumors.