贝母辛抗大鼠肝纤维化的作用研究

目的 观察贝母辛对四氯化碳（CCl₄）致大鼠肝纤维化的保护作用。方法 实验设对照组，模型组，贝母辛低、中、高剂量（2.5、5、10 mg/kg）组。除对照组外，其他组大鼠每隔3天ip给予CCl₄ 1次，连续8周，贝母辛各组于造模第4周开始给药，每天1次，给药至造模结束后4周。通过观察肝纤维化大鼠肝组织病理学变化，血清中谷丙转氨酶（ALT）、谷草转氨酶（AST）、碱性磷酸酶（ALP）、谷氨酰氨基转移酶（GGT）、透明质酸（HA）、层黏连蛋白（LN）、III型前胶原（PC-III）、IV型胶原（IV-C）水平，以及肝组织中羟脯氨酸（Hyp）、超氧化物歧化酶（SOD）、丙二醛（MDA）量的变化，考察贝母辛对肝纤维化大鼠的影响。结果 与模型组相比，贝母辛组肝纤维化大鼠肝病理组织学改变得到明显改善，血清中ALT、AST、ALP、GGT、HA、LN、PC-III、IV-C水平显著降低（P＜0.05、0.01）；肝组织中Hyp、MDA的量显著减少（P＜0.01），SOD的量增加（P＜0.01）。结论 贝母辛对CCl₄致大鼠肝纤维有较好的保护作用，其机制可能与抑制肝内胶原合成、降低自由基生成、减轻脂质过氧化有关。

Protection of peimisine on hepatic fibrosis of rats induced by CCl4

Objective To investigate the protective effect of peimisine on carbon tetrachloride (CCl₄)-induced hepatic fibrosis in rats. Methods The rats were divided into control, model, low-, mid-, and high-dose (2.5, 5, and 10 mg/kg) peimisine groups. Hepatic fibrosis models were induced by ip injection of CCl₄ in rats once every 3 d for 8 weeks. The rats in the treatment groups were administered four weeks after the model establishment, once daily until the end of the week 4 after the model establishment. The levels of serum alanine aminotransferase (ALT), aspartate aminotransferase (AST), alkaline phosphatase (ALP), glutamyl transpeptidasecc (GGT), hyaluronie acid (HA), laminin (LN), type III procollagenc (PC-III), and collegen type IV (IV-C) were assayed, and hepatic tissue contents of hydroxyprolinc (Hyp), superoxide dismutase (SOD), and malondialdehyde (MDA) were determined. The effect of peimisine on hepatic fibrosis in rats was observed. Results Compared with the model control group, the hepatic fibrosis of rats in peimisine groups was improved obviously, the levels of ALT, AST, ALP, and GGT in serum were lowered obviously (P < 0.05, 0.01), also the serum levels of HA, LN, PC-III, IV-C, and the contents of Hyp and MDA in liver tissue were decreased (P < 0.01), while the level of SOD was increased (P < 0.01). Conclusion Peimisine has the protective effect on the experimental hepatic fibrosis formation. The possible mechanisms are associated with inhibiting fibrogenesis and fibrosis accumulation, and decreasing lipid peroxidation.