成纤维细胞生长因子受体各亚型与小鼠肺纤维化及衰老的关系

目的观察成纤维细胞生长因子受体（FGFR）各亚型与肺组织的纤维化及衰老之间的关系。方法应用荧光定量聚合酶
链反应方法，分别检测不同鼠龄的小鼠肺组织FGFR1-4的表达水平；同时，采用常规病理切片（HE染色和Masson染色）观察不
同鼠龄小鼠肺组织的纤维化状况。结果（1）FGFR各亚型在小鼠肺组织中表达水平不均匀，FGFR2的含量高于其他几个亚型；
（2）8月龄小鼠肺组织中FGFR亚型表达水平均显著低于5周龄小鼠肺组织中FGFR的表达；（3）8月龄的小鼠肺组织表现出肺纤
维化的变化。结论小鼠肺组织中FGFR水平的下降可能与年龄的增加相关；不同年龄组均以FGFR2的含量为高；小鼠肺组织
随着鼠龄增加呈现不同程度的纤维化，可能与FGFR的表达下降相关；提示FGFs/FGFR可能参与衰老的发生及肺纤维化调节。

Pulmonary expression levels of fibroblast growth factor receptors and lung fibrosis in mice at different ages

Objective To explore the correlation of pulmonary expressions of fibroblast growth factor receptors (FGFR1-4) with
lung fibrosis and aging. Methods Real-time fluorescence quantitative PCR was used to detect the expression levels of FGFR1-4
in the lung tissues, and lung fibrosis was observed by HE and Masson staining in mice at different ages. Results The 4
subtypes of FGFR showed different expression levels in the lung tissues of mice, and FGFR2 had the highest expressions. The
expression levels of all the 4 FGFR subtypes in 8-month-old mice were significantly lower than those in 5-week-old mice. The
8-month-old mice tended to present with histological changes of lung fibrosis. Conclusion FGFR expressions is
down-regulated with aging in mice. Among the FGFR subtypes, FGFR2 is expressed at the highest level. The occurrence of
lung fibrosis with aging is probably associated with down-regulated FGFR expression. FGF/FGFR signaling may participate in
the aging process and regulation of lung fibrosis.