牡蛎中诺如病毒核酸两种提取方法比较

目的寻找简便有效的更适合长期监测的牡蛎中诺如病毒核酸提取的方法。方法通过甘氨酸聚乙二醇富集后硅胶膜离心
柱法（方法A）和蛋白酶K生物消化后顺磁性硅胶基法（方法B）两种核酸提取方法，采用实时RT-PCR检测自然状态的牡蛎和人
工染毒状态的牡蛎来进行比较。结果两种方法的阳性检出率没有差异，总体比较方法B的病毒回收率高。结论方法B优于
方法A，且方法B更快捷，故方法B更适合长期监测。

Comparison of two nucleic acid extraction methods for norovirus in oysters

Objective To explore a convenient and effective method for norovirus nucleic acid extraction from oysters suitable
for long-term viral surveillance. Method Two methods, namely method A (glycine washing and polyethylene glycol
precipitation of the virus followed by silica gel centrifugal column) and method B (protease K digestion followed by
application of paramagnetic silicon) were compared for their performance in norovirus nucleic acid extraction from oysters.
Real-time RT-PCR was used to detect norovirus in naturally infected oysters and in oysters with induced infection. Results
The two methods yielded comparable positive detection rates for the samples, but the recovery rate of the virus was higher
with method B than with method A. Conclusion Method B is a more convenient and rapid method for norovirus nucleic acid
extraction from oysters and suitable for long-term surveillance of norovirus.