| 均匀设计与正交设计在钩吻生物总碱提取工艺筛选研究中的应用 |
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| 引用本文： | 张兰兰,黄昌全,张忠义,林敬明.均匀设计与正交设计在钩吻生物总碱提取工艺筛选研究中的应用[J].南方医科大学学报,2003,23(9):914-915,924. |
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| 作者姓名： | 张兰兰 黄昌全 张忠义 林敬明 |
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| 作者单位： | 第一军医大学珠江医院药剂科,广东,广州,510282 |
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| 基金项目： | 广东省科技攻关项目(2001年度C30801)~~ |
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| 摘 要： | 目的优选简便高效的钩吻生物总碱的提取工艺,考察不同提取条件下的提取率。方法分别用均匀设计和正交设计两种方法对钩吻生物总碱的提取工艺中溶剂种类、提取系统中固液比例和回流时间3个因素进行优选。结果两种方法的结果均为采用氯仿作为溶剂、固液比例为1.0∶7.5、回流时间3 h×3,所得总碱含量最高。结论正交设计与均匀设计试验所得结果相近,结论基本一致。
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| 关 键 词： | 均匀设计 正交设计 钩吻总碱 提取工艺 |
| 文章编号： | 1000-2588(2003)09-0914-02 |
| 修稿时间： | 2003年5月12日 |
Comparative study of uniform design and orthogonal design in the extraction of Gelsemium alkaloids |
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| ZHANG Lan-lan,HUANG Chang-quan,ZHANG Zhong-yi,LIN Jing-ming.Comparative study of uniform design and orthogonal design in the extraction of Gelsemium alkaloids[J].Journal of Southern Medical University,2003,23(9):914-915,924. |
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| Authors: | ZHANG Lan-lan HUANG Chang-quan ZHANG Zhong-yi LIN Jing-ming |
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| Institution: | ZHANG Lan-lan,HUANG Chang-quan,ZHANG Zhong-yi,LIN Jing-ming Department of Pharmaceutics,Zhujiang Hospital,First Military Medical University,Guangzhou 510282,China |
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| Abstract: | Objective To develop a simple and efficient method for extracting Gelsemium alkaloids. Methods Uniform de-sign and orthogonal design were respectively utilized to optimize the 3 factors in the extraction process, namely the type of solvent used, the solid-to-liquid ratio in the extraction system and the reflux time. Results Both of the design methods adopted trichloromethane as the solvent, with the solid-to-liquid ratio of 1:7.5 and reflux time of 3 h with 3 repetitions for the highest yield of alkaloids. Conclusion Similar results are obtained from the two design methods for extracting Gelsemium alkaloids. |
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| Keywords: | uniform-design orthogonal-design Gelsemium elegans Benth alkaloid/extraction |
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