NPY和NT在子痫前期患者胎盘组织中的表达及意义

目的检测子痫前期患者胎盘组织中神经肽Y（NPY）及神经降压素（NT）表达的变化。探讨妊娠期高血压疾病的发病机制。方法采用亲合免疫细胞化学技术链霉素-生物素-过氧化物酶复合物技术（SABC法）对30例正常妊娠妇女（正常妊娠组），30例轻度子痫前期组，30例重度子痫前期组患者胎盘组织中的NPY及NT进行测定。结果各组胎盘组织中均有NPY和NT的表达。随着妊娠高血压疾病病情的加重，胎盘组织中NPY的表达逐渐升高。轻度子痫前期NPY表达强度显著高于正常妊娠组，差异有统计学意义（X^2=2．06，P〈0．05），重度子痫前期组NPY表达强度显著高于轻度子痫前期组，差异有统计学意义（X^2=2．25，P〈0．05）；重度子痫前期组NT的表达弱于正常妊娠组，差异有统计学意义（X2=2．34，P〈0．05），重度子痫前期NT的表达弱于轻度子痫前期组，差异有统计学意义（X^2=2．10，P〈0．05），轻度子痫前期与正常妊娠组NT表达强度比较差异无统计学意义（X^2=1．16，P〉0．05）。结论NPY与NT在胎盘组织中的表达情况与妊娠高血压疾病的轻重程度密切相关。

Expression and significance of NPY and NT in placenta of pregnant women with pre-eclampsia

Objective To study the expression change of neuropeptide Y （NPY） and neurotesin （NT） in placenta of pregnant women with pre-eclampsia and to investigate the pathogenesis of hypertensive disorder complicating pregnancy. Methods SABC was used to determine the NPY and NT in placental tissure samples of 30 healthy pregnant women （ control group）, 30 mild pre-eclampsia cases （ mild preeclampsia group） and 30 severe pre-eclampsia cases （ severe pre-eclampsia group）. Results Both NPY and NT were expressed in placenta tissues of each group. The expression of NPY increased with the progress of hypertensive disorder complicating pregnancy. The expression of NPY in mild pre-eclampsia group was significantly higher than that in normal pregnant women, and the difference was statistically significant （X^2 = 2.06 ,P 〈 0.05 ）, and the expression of NPY in severe pre-eclampsia group was significantly higher than that in mild pre- eclampsia group, and the difference was significant （X^2 = 2.25, P 〈 0.05 ）. The expression of NT in patients with severe pre-eclampsia was significantly reduced compared with that in normal pregnant women, and it was lower in patients with severe pre-eclampsia than that in patients with mild pre-eclampsia. The differences were significant （X2 value was 2.34 and 2.10, respectively, both P 〈 0.05）. There was no significant difference in NT expression between mild pre-eclampsia group and normal group （X^2 = 1.16, P 〉 0.05 ）. Conclusion Abnormal expression of NPY and NT in placenta tissue is closely related with the severity of hypertensive disorder complicating pregnancy.