不同周龄C57-ras转基因小鼠模型杂交1代CB6F1小鼠的脏器及血液学参数测定

目的 测定4周龄和8周龄不同性别CB6F1小鼠的脏器及血液生理生化指标正常值, 并比较周龄、性别对各项指标的影响。方法 分别取20只雌雄各半的4周龄和8周龄的CB6F1小鼠, 活体称重, 解剖称取主要脏器重量,采血测定血液生理指标和血清生化指标。结果 4周龄和8周龄CB6F1在体重、心、肝、脾、右卵巢、左睾丸、右睾丸、白细胞计数(WBC)、红细胞计数(RBC)、血红蛋白浓度(HGB)、红细胞压积(HCT)、平均红细胞体积(MCV)、平均红细胞血红蛋白(MCH)、血小板压积(PCT)、平均血小板体积(MPV)、血小板分布宽度(PDW)、淋巴细胞(LYM)、总蛋白(TP)、丙氨酸氨基转移酶(ALT)、白蛋白(ALB)、磷(P)、甘油三酯(TG)这22项指标上差异存在显著性(P<0.01), 在左肾、右肾、右肾上腺、胸腺、左卵巢、红细胞分布宽度(RDW)、单核细胞百分比(MON%)、尿素(BUN)这8项指标上差异有统计学意义(P<0.05)。从性别上比较, 4周龄CB6F1在体重、心、肝、脾、肺、左肾、右肾、平均红细胞血红蛋白浓度(MCHC)、LYM、ALT、ALP、葡萄糖(GLU)、P、总胆固醇(CHO)这14项指标上都有雌雄间的显著差异(P<0.01), 在右肾上腺、WBC、PCT、平均血小板体积(MPV)、总蛋白(TP)、BUN这6项指标上存在雌雄间的统计学差异(P<0.05);8周龄CB6F1在体重、心、肝、肺、左肾、右肾、MCV、PCT、LYM、淋巴细胞百分比(LYM%)、中性粒细胞百分比(NEUT%)、ALT、GLU、P、CHO这15项指标上都有雌雄间的显著差异(P<0.01), 在WBC、RBC、MPV、中性粒细胞(NEUT)、TP这5项指标上存在雌雄间的统计学差异(P<0.05)。结论 本文测定了不同周龄不同性别CB6F1小鼠的脏器及血液生理生化指标, 为其建立正常检测指标和应用提供参考。

Determination of hematological and visceral weight parameters of F1 carcinogenesis transgenic C57-ras model CB6F1 mice

Objective To determine the normal range of hematological and visceral weight parameters of F1 4-week and 8-week old, male and female transgenic CB6F1 mice. The influence of gender and week age on the biochemical parameters was assessed. Methods 4-week and 8-week old CB6F1 mice, half male and half female (n=20 in each group), were weighed alive, dissected to weigh the main viscera, and blood samples were collected to test the physiological and biochemical parameters. Results When 4-week old and 8-week old CB6F1 mice were compared, there were significant differences in 22 parameters (body weight, heart, liver, spleen, left ovary, left testis, right testis, WBC, RBC, HGB, HCT, MCV, MCH, PCT, MPV, PDW, LYM, TP, ALT, ALB, P and TG) (P<0.01 for all), and in 8 parameters (left kidney, right kidney, right adrenal, thymus, left ovary, RDW, MON% and BUN) (P<0.05 for all). When male and female 4-week CB6F1 mice were compared, there were significant differences in 14 parameters (body weight, heart, liver, spleen, lung, left kidney, right kidney, MCHC, LYM, ALT, ALP, GLU, P and CHO) (P<0.01 for all), and in 6 parameters (right adrenal, WBC, PCT, MPV, TP and BUN) (P<0.05 for all). For male and female 8-week old CB6F1 mice, there were significant differences in 15 parameters (body weight, heart, liver, lung, left kidney, right kidney, MCV, PCT, LYM, LYM%, NEUT%, ALT, GLU, P and CHO) (P<0.01 for all), and in 5 parameters (WBC, RBC, MPV, NEUT and TP) (P<0.05 for all). Conclusions The normal range of hematological and visceral weight parameters of 4-week and 8-week old male and female CB6F1 mice are determined. Our study establishes normal detection indexes of CB6F1 mice and provides useful reference for its application.