湖北省中华按蚊对溴氰菊酯的抗药性监测

目的了解中华按蚊对溴氰菊酯抗药性现状，为合理使用杀虫剂提供科学依据。方法采用WHO的成蚊滤纸接触法，观察首只蚊虫击倒时间、击倒率和死亡率，以区分剂量判定抗性级别。结果江陵县、襄州区、枣阳市、通城县、天门市、夷陵区、武穴市、广水市、曾都区、京山县和恩施市被溴氰菊酯击倒的首只中华按蚊时间分别为6、20、ll、9、20、6、30、5、14、25和23min，60min时的击倒率（％）分别为9．62、7．69、14．55、2．11、3．00、48．94、2．80、10．78、14．00、1．00和3．06；11个地区中华按蚊在溴氰菊酯区分剂量作用下的死亡率除恩施市为70．85％外，其余均〈50．00％，为高抗群体。结论湖北区域范围内中华按蚊对溴氰菊酯的抗击倒力强，抗性程度高，抗性发展快，应加强监测，现场应用宜适当加大使用剂量，确保防制效果。

Monitoring of resistance of Anopheles sinensis to deltamethrin in Hubei province,China

Objective To investigate the resistance of Anopheles sinensis to deltamethrin and provide baseline data for proper use of insecticide. Methods The filter paper contact method for adult mosquitoes, as recommended by WHO, was used to determine the time for initial knockdown, knockdown rate, and mortality of An. sinensis, and the levels of resistance were determined based on the discriminating doses. Results For the An. sinensis in Jiangling county, Xiangzhou district, Zaoyang city, Tongcheng county, Tianmen city, Yiling district, Wuxue city, Guangshui city, Zengdu district, Jingshan county, and Enshi city, the time for initial knockdownby dehamethrin was 6, 20, 11, 9, 20, 6, 30, 5, 14, 25, and 23 min, respectively; the knockdown rate caused by deltamethrin at 60 min was 9.62%, 7.69%, 14.55%, 2.11%, 3.00%, 48.94%, 2.80%, 10.78%, 14.00%, 1.00%, and 3.06%, respectively. The mortality of An. sinensis at the discriminating dose of deltamethrin was less than 50.00% in all sampling areas except for Enshi where the mortality was 70.85%. Conclusion The resistance of An. sinensis to deltamethrin in Hubei province was high and the spreading of resistance was rapid. Thus, we should strengthen the monitoring of insecticide resistance and implement strategic dosing of insecticide to achieve better control effect.