基质金属蛋白酶在曝光及非曝光部位皮肤中的表达

目的 检测基质金属蛋白酶－1、3、9（MMP-1、3、9）在曝光部位和非曝光部位皮肤中的表达，探讨它们在皮肤光老化机制中的作用。方法 应用免疫组化方法分别对23例女性健康志愿者前臂伸侧（曝光）和上臂内侧（非曝光部位）皮肤石蜡标本中的MMP-1、MMP-3、MMP-9进行检测，表达强度以免疫反应强度分布指数（IRIDI）表示，采用Wilcoxon符号秩和检验、Mann-Whitney秩和检验及Spearman秩相关分析进行统计学处理。结果 MMP-1、MMP-3、MMP-9在曝光和非曝光部位均表达，曝光部位皮肤组织中MMP-1、MMP-3、MMP-9的IRIDI均值（范围）分别为7.70（3 ～ 12）、9.22（6 ～ 12）、8.30（6 ～ 12），非曝光部位分别为4.26（2 ～ 6）、5.39（2 ～ 9）、4.04（1 ～ 6），曝光部位MMP-1、MMP-3、MMP-9的IRIDI均显著高于非曝光部位（均P < 0.01）。50岁以上组曝光部位皮肤组织MMP-1、MMP-3、MMP-9的IRIDI分别为9.17（6 ～ 12）、10.58（8 ～ 12）、8.92（8 ～ 12），非曝光部位分别为4.75（2 ～ 6）、6.42（4 ～ 9）、4.33（3 ～ 6），曝光部位均显著高于非曝光部位（P < 0.05）；50岁以下组曝光部位皮肤组织MMP-1、MMP-3、MMP-9的IRIDI分别为6.09（3 ～ 8）、7.73（6 ～ 9）、7.64（6 ～ 12），非曝光部位分别为3.73（2 ～ 6）、4.27（2 ～ 8）、3.73（1 ～ 6），曝光部位均显著高于非曝光部位（P < 0.05）；50岁以上组曝光部位显著高于50岁以下组，而两组非曝光部位皮肤组织MMP-1、MMP-9的IRIDI值差异无统计学意义（P > 0.05）。曝光部位皮肤组织MMP-1、MMP-3、MMP-9的IRIDI值与年龄呈正相关（r = 0.66、0.69、0.74，P < 0.01），非曝光部位皮肤组织MMP-1、MMP-9的IRIDI值与年龄无相关性。结论 MMP-1、MMP-3、MMP-9在曝光部位表达显著高于非曝光部位，三者在光老化的发生发展中均起作用，但作用机制可能不同。

Expression of matrix metalloproteinases in sun-exposed and-unexposed skin

Objective To study the expression of matrix metalloproteinase(MMP)-1,-3 and-9 in sun-exposed and-unexposed skin as weil as its significance in the mechanism of skin photoaging.Methods Skin samples were resected from the exmnsor side of forearm(sun-exposed area)and flexor side of upper arm(sun-unexposed area)of 23 healthy female volunteers.The expression of MMP-1,-3 and-9 was detected by immunohistochemical staining in 46 skin samples.Immunoreactive intensity distribution index (IRIDI)was calculated to assess the expression of MMP-1,-3 and-9.Wilcoxon signed ranks test,Mann-Whitney U-test and Spearman rank correlation analysis were performed.Results MMP-1,-3 and-9 were expressed in both sun-exposed and -unexposed skin.The average IRIDI value for MMP-1,-3 and-9 was 7.70(range,3 to 12).9.22(range,6 to 12),8.30(range,6 to 12)in sun-exposed skin,and 4.26 (range,2 to 6),5.39(range,2 to 9),4.04(range,1 to 6)in sun-unexposed skin,respectively;significant difierence existed between sun.exposed and-unexposed skin in the three parameters(all P＜0.01).A significant inerease was observed in the average IRIDI value for MMP-1,-3 and-9 in sun-exposed skin vs.sun-unexposed skin in women above 50 years of age (9.17 vs 4.75,10.58 vs 6.42,8.92 vs 4.33,respectively,all P＜0.05).In women younger than 50 years,the average IRIDI value for MMP-1,-3 and-9 was 6.09(range,3 to 8),7.73(range,6 to 9),7.64(range,6 to 12)in sun-exposed skin,significantly higher than that in sun-unexposed skin[3.73(range,2 to 6),4.27(range,2 to 8),3.73(range,1 to 6),all P＜0.05].Increased IRIDI scores of MMP-1,-3 and -9 were noticed in sun-exposed skin in women above 50 years of age vs.those younger than 50 years.but there was no statistical difrerence in MMP-I or MMP-9 between the two aged groups in sun-unexposed skin(all P＞0.05).The IRIDI scores of MMP-1,MMP-3 and MMP-9 were positively correlated with age(r=0.66,0.69,0.74,all P＜0.01)in sun-exposed skin,but the IRIDI scores of MMP-1 and MMP-9 uncorrelated with age in sun-unexposed skin.Conclusions There isan elevated expression of MMP-1,-3 and.9 in sun-exposed skin VS.SUn.unexposed skin.hinting that these three MMPs play a role in the occurrence and development of photoaging,but their biological mechanism may be different.