| 补肾阴对胚胎干细胞活动的影响 |
| |
| 引用本文： | 安红梅,胡兵,史云峰,许丽雯,史秀峰,陈方敏,顾耘,潘露茜,王志.补肾阴对胚胎干细胞活动的影响[J].中国中医药信息杂志,2007,14(6):24-26. |
| |
| 作者姓名： | 安红梅 胡兵 史云峰 许丽雯 史秀峰 陈方敏 顾耘 潘露茜 王志 |
| |
| 作者单位： | 1. 上海中医药大学附属龙华医院,上海,200032
2. 上海中医药大学,上海,201203 |
| |
| 摘 要： | 目的观察补肾阴中药复方对胚胎干细胞活动的影响。方法选用胚胎干细胞模型CRL-1825细胞株,加入补肾阴中药复方大鼠含药血清,观察补肾阴中药对干细胞分化、凋亡、细胞周期及其关键基因表达的影响。结果补肾阴中药复方含药血清可以抑制干细胞的分化和凋亡,促进干细胞增殖以及细胞周期的进程,促进干细胞Wnt、Oct4等基因的表达,下调P16INK4a基因的表达。结论补肾阴中药复方可以促进干细胞增殖、维持其不分化状态。
|
| 关 键 词： | 补肾阴 中药 胚胎干细胞 |
| 文章编号： | 1005-5304（2007）06-0024-03 |
| 收稿时间： | 2006-10-16 |
| 修稿时间： | 2006-10-16 |
Effects of Tonifying Kidney-Yin on the Activities of Embryonic Stem Cell |
| |
| AN Hong-Mei, HU Bing, SHI Yun-Feng, et al.Effects of Tonifying Kidney-Yin on the Activities of Embryonic Stem Cell[J].Chinese Journal of Information on Traditional Chinese Medicine,2007,14(6):24-26. |
| |
| Authors: | AN Hong-Mei HU Bing SHI Yun-Feng |
| |
| Institution: | Longhua Hospital, Shanghai University of TCM, Shanghai 200032, China |
| |
| Abstract: | Objective To study the effects of tonifying kidney-yin on activities of embryo stem cell. Methods Embryonic stem cell line CRL-1825 was choosed as model cell in present study, and tonifying kidney-yin herb serum was added to CRL-1825 cell. The influence of tonifying kidney-yin on stem cell differentiation, cell cycle, apotosis and express of key genes were observed. Results Tonifying kidney-yin can inhibit stem cell differentiation and apotosis, promote stem cell proliferation and progression of cell cycle. In addition, tonifying kidney-yin can up-regulate Wnt, Oct4 gene expression, and down-regulation P16INK4a expression. Conclusions Tonifying kidney-yin can promote stem cell proliferation and maintain the state of stem cell. |
| |
| Keywords: | Tonifying the kidney-yin Chiese herb embryo stem cell |
| 本文献已被 CNKI 维普 万方数据 等数据库收录！ |
|
