改良型三血管结扎慢性脑缺血模型大鼠的行为学评价

目的 检测改良三血管结扎慢性脑缺血模型(m-3VO)与舣侧劲总动脉结扎(2VO)模型的空间学习记忆能力、探索能力与抑郁表现,以评价m-3VO改良模型的行为学表现.方法 按照3VO改良术式与2VO经典术式分别制造m-3VO模型、2VO模型以及假于术对照模型.术后第8周进行Morris水迷宫、旷场实验和强迫游泳实验检测.结果 Morris水迷宫检测显示m-3VO组第4,5天潜伏期(37.69±5.11)s,(32.55±4.28)s]与假手术对照组(18.46±2.63)s,(15.9±2.16)s]相比时间较长(t=3.61,P<0.01;t=3.028,P<0.05),2VO组第4,5天潜伏期(42.94±3.05)s,(37.43±4.55)s]与假手术对照组相比时间较长(t=4.72,P<0.001;t:4.153,P<0.001),m-3VO组(7.72±1.92)s]与2VO组(7.15±2.66)s]的NE象限游泳时间亦比假手术对照组时间(11.19±2.137)s]长(q=2.98,P<0.05;q=3.81,P<0.01);旷场实验榆测显示2VO水平运动得分(83.25±13.53)分]比假手术对照组(55.6±16.8)分]高(q=3.27,P<0.01);强迫游泳实验榆测显示m-3VO组、2VO组静止次数、游泳次数和攀爬次数与假手术对照组相比,均差异无显著性(P>0.05).结论 m-3VO模型大鼠存术后8周出现空间学习记忆能力的损害,而探索能力无明显改变且未出现抑郁,其行为学表现与2VO模型大鼠相似.

The ethology representation of the modified three-vessel-occlusion rat model of chronic cerebral hypoperfusion

Objective To estimate the ethology representation of the modified model of chronic cerebral hypoperfusion ( m-3VO) , created by occluding bolh common carotid in combination with one vertebral artey. Methods Rats were randomly put into m-3VO group,2VO group,and sham group ,and were subjected to the matching operation. Eight weeks after the operation, Morris water maze,open field test,and forced swimming lest were carried out. Results Compared with the sham group (18.46 ± 2. 63)s, (15. 9 ± 2. 16)s] .escape latencies of Morris water maze of m-3VO group ( 37. 69 ± 5. 11 ) s, ( 32. 55 ± 4. 28 ) s] and 2V0 group (42. 94 ± 3.05)s, (37.43 ± 4. 55)s] in the fourth and fifth days significantly increased (t = 3. 61 , P<0. 01 ; l = 3. 028, P<0.05 ; t = 4.72, P< 0. 001 ; t = 4. 153, P<0.001). The time of m-3VO group (7. 72 ± 1. 92) s] and 2VO group (7. 154 ± 2.66)s] spent in NE square was also longer than that of the sham group(11. 19 ± 2. 137)s] (q = 2.98, P< 0.05; q = 3.81 , P< 0.01 ). And the crossing score of open field test of 2VO group(83. 25 ± 13. 53) was higher than that of the sham group (55. 6 ± 16. 8) ( q = 3. 27, P < 0. 01 ) , but immobility times, swimming times and climbing times of forced swimming test were not different between the three groups (P>0. 05). Conclusion The spatial learning ability of m-3V0 group rats are impaired 8 weeks after the operation, which is similar to that of 2VO group rats,and the m-3V0 operation have few effect on exploring ability and depression of rats.