中药胃康宁对胃癌细胞周期因子Cyclin E、CDK2和p27表达的影响

目的:观察中药胃康宁含药血清对胃癌细胞Cyc lin E、CDK2(Cyc lin dependant k inase 2,CDK2)和p27mRNA及其蛋白表达的作用。方法:分别以高、中、低不同剂量的胃康宁制剂对W istar雄性大鼠进行灌胃,制备含药血清,然后分别用不同剂量组含药血清培养胃癌细胞,采用免疫组织化学SABC法检测各组胃癌细胞中Cyc linE、CDK2和p27蛋白的表达,RT-PCR法检测各组胃癌细胞中Cyc lin E、CDK2和p27 mRNA的表达。结果:免疫组化法结果显示,胃康宁高、中、低剂量组Cyc lin E和CDK2灰度值均有明显升高,与空白对照组比较,P<0.05~0.01,而p27灰度值则显著降低(P<0.05~0.01);RT-PCR结果显示,与空白对照组比较,胃康宁低、中、高剂量组均能明显降低Cyc lin E和CDK2 OPTD I值(P<0.05~0.01)。同时,还能升高p27 OPTD I值(P<0.05~0.01)。结论:中药胃康宁可抑制胃癌细胞周期因子Cyc lin E、CDK2及其mRNA的表达,促进细胞周期抑制因子p27及其mRNA的表达,这可能是胃康宁抑制胃癌细胞生长的机制之一。

Effect of Weikangning Decoction on Expression of Cyclin E, CDK2, and p27

Objective: To observe the effect of containing drugs serum of weikangning decoction(WKN) on expression of Cyclin E,CDK2(Cyclin dependant kinase 2,CDK2) and p27 in gastric cancer cell line MGC-803.Methods: Total of 120 male Wistar rats were divided into control group,high dose group,medium dose group and low dose group fed with natural saline,20,10,and 5 g/kg of WKN,respectively.The experimental animals were finally killed for the preparation of drug-containing serum.The gastric cancer cell MGC-803 was cultured with the drug-containing serum drawn from the rats in different groups.The expression of Cyclin E,CDK2 and p27 was detected with immunohistochemistry method-SABC.The expression of mRNA of Cyclin E,CDK2 and p27 were detected with RT-PCR. Results: In high,medium and low dose groups,the gray scales of cyclin E,CDK2 and the OPDTI values of p27 increased significantly,but the gray scales of p27 and the OPDTI values of cyclin E,CDK2 decreased remarkble,compared with that in control group.Conclusion: The decoction of WKN can decrease the expression of Cyclin E,CDK2,increases the expression of p27.This effect may be involved in mechanism of gastric cancer cell growth inhibition induced by WKN.