高效液相色谱法同时测定黄氏响声丸中5种蒽醌类成分含量

目的:建立黄氏响声丸中5种蒽醌类活性成分的高效液相色谱含量测定方法,有利于制剂的质量控制.方法:采用高效液相色谱法,色谱柱:Shimadzu VP-ODS柱(4.6mm×250mm,5μm),流动相为乙腈-0.1%磷酸梯度洗脱,流速为1.0ml/min,检测波长为254nm,柱温30℃.结果:5种成分为芦荟大黄素0.0...

Simultaneous determination of five anthraquinone constituents in Huangshixiangsheng Pills by high performance liquid chromatography

Objective：To establish an HPLC method for determining five anthraquinones contents in Huangshixiangsheng Pills,and make it used for the quality control of Huangshixiangsheng Pills.Methods：HPLC was carried out on Shimadzu VP-ODS（4.6mm×250mm,5.0μm） with acetonitrile-0.1% phosphoric acid in gradient elution.The flow rate was 1.0 mL·min-1,the detection wavelength was 254 nm and the column temperature was 30℃.Results：Five anthraquinones had good linear relationship：Aloe-emodin in the range of 0.042～0.840 μg（r=0.9996）,rhein in the range of 0.168～3.352 μg（r=0.9998）,emodin in the range of 0.084～1.680 μg（r=0.9997）,chrysophanol and physcion in the range of 0.093-1.852 μg（r=0.9999） and 0.174～3.488 μg（r=0.9994）.Their average recoveries were 99.8%（RSD=1.34%,n=5）,99.8%（RSD=1.72%,n=5）,100.0%（RSD=0.89%,n=5）,99.5%（RSD=0.97%,n=5）,and 100.2%（RSD=1.04%,n=5）,respectively.Conclusion：This method is rapid,simple and accurate and it can be used for the determination of aloe-emodin,rhein,emodin,chrysophanol and physcion in Huangshixiangsheng Pills simultaneously.