| PCR-RFLP技术在甲醛固定石蜡包埋组织中的应用 |
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| 引用本文： | 李伟,单海燕,阚卫军,张明阳,王东林. PCR-RFLP技术在甲醛固定石蜡包埋组织中的应用[J]. 中国交通医学杂志, 2011, 25(1): 31-32,35 |
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| 作者姓名： | 李伟 单海燕 阚卫军 张明阳 王东林 |
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| 作者单位： | 李伟,LI Wei(苏州大学附属儿童医院检验科,江苏,215000);单海燕,SHAN Haiyan(南通大学附属医院普外科);阚卫军,KAN Weijun(南通市公安局刑警支队);张明阳,王东林,ZHANG Mingyang,Wang Donglin(南通大学医学院法医学教研室) |
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| 基金项目： | 南通大学医学院科研基金研究项目 |
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| 摘 要： | 目的:探讨mtDNA分析技术在甲醛固定石蜡包埋组织中的应用.方法:应用限制酶RsaI和MnlI消化mtDNA D-LOOP区(HVI16106-16297)PCR扩增产物,聚丙烯凝胶电泳进行RFLP分型的方法,对随机个体血液样品150例.正常组织10例和甲醛固定石蜡包埋组织5例进行检验.结果:在150例随机个体中,Rs...
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| 关 键 词： | 甲醛固定石蜡包埋组织 线粒体DNA 聚合酶链反应-限制性片段长度多态性技术 |
The application of PCR-RFLP technology in Formalin-Fixed and Paraffin-Embedded Samples |
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| LI Wei,SHAN Haiyan,KAN Weijun,ZHANG Mingyang ,Wang Donglin. The application of PCR-RFLP technology in Formalin-Fixed and Paraffin-Embedded Samples[J]. Chinese Medical JOurnal of Communications, 2011, 25(1): 31-32,35 |
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| Authors: | LI Wei SHAN Haiyan KAN Weijun ZHANG Mingyang Wang Donglin |
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| Affiliation: | LI Wei1,SHAN Haiyan2,KAN Weijun3,ZHANG Mingyang 4,Wang Donglin4(1Children's Hospital Affiliated of Suzhou University,Suzhou 215003,2Department of General Surgery,Affiliated Hospital of Nantong University,3Criminal Police Detachment of Nantong Public Security Bureau,4Department of Forensic Medicine,Nantong University) |
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| Abstract: | Objective:To study the application of RFLP technology in FFPE.Methods:The high variable region 16106-16297 in D-LOOP was amplified by PCR.150 unrelated Han individuals,10 normal tissue and 5 cases of formalin-fixed paraffin-embedded tissues were investigated and analyzed by RFLP.Results:In 150 unrelated Han individuals,3 genotypes were found by RsaI-RFLP technology;8 genotypes were found by MnlI-RFLP technology.Detected in the tissues,all typing results were consistent.Conclusion:PCR-RFLP is suitable for an... |
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| Keywords: | Formalin-fixed paraffin-embedded tissues mtDNA polymerase chain reaction restriction fragment length polymorphism RsaI MnlI |
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