HPLC 法测定乐脉胶囊中芍药苷和丹酚酸 B 的含量

目的建立HPLC法测定乐脉胶囊中芍药苷和丹酚酸B的含量。方法芍药苷和丹酚酸B均采用C18（4．6mm×250mm，5μm）色谱柱；流动相分别为乙腈-0．05％磷酸（13：87）和乙腈-甲醇-1．7％甲酸溶液（4：27：69）；检测波长分别为230和286nm，柱温为30℃，流速为1．0ml·min^-1。结果芍药苷在0．20403—4．0806μg范围内得芍药苷回归方程Y=1×10^6X＋2631（r=0．9999）；丹酚酸B在0．12573～2．5147μg范围内得回归方程Y=937308X＋560947（r=0．9996），两组分分别与峰面积呈良好的线性关系。芍药苷的平均回收率为98．9％，RSD为1．8％；丹酚酸B平均回收率为100．4％，RSD为2．8％。结论该方法结果准确，重复性好，可作为乐脉胶囊质量控制的定量方法。

Determination of Paeoniflorin and Salvianolic acid B in Lemai Capsules by HPLC

Objective To establish an HPLC method for the determination of paeoniflorin and salvianolic acid B in Lemai capsules. Methods Chromatographic experiments on paeoniflorin were performed on Cls column, the mobile phase consisted of acetonitrile-0.05% phosphoric acid （13： 87） ,the detection wavelength was 230 nm,the column temper-ature was 30 ℃ ,and the flow rate was 1.0 ml ·min^-1. Similarly,chromatographic experiments on salvianolic acid B were performed on C18 column （4. 6 mm × 250 mm, 5 μm）, the mobile phase was aeetonitrile-methanol-1.7% formic acid （4：27：69） ,the detection wavelength was 286 nm, the column temperature was 30 ℃, and the flow rate was 1.0 ml·min^ - 1. Results The linear equations were Y = 1 × 10^6 X ＋ 2631 （ r = 0. 9999） for paeoniflorin and Y = 937 308 X ＋ 560 947 （ r = 0. 9996） for salvianolic acid B, respectively. The linear range was 0.204 03 - 4.0806 μg for paeoniflorin and 0. 201 10 - 2. 5147 μg for salvianolie acid B, respectively. The average recovery was 98.9% （ RSD = 1.8% ） for paeoniflorin and 100.4% （ RSD = 2.8% ） for salvianolic acid B, respectively. Conclusion This method is simple and accurate with good repeatability. It can be used for quantity control of Lemai capsules.