Th1和Th2型细胞因子对小鼠感染血吸虫的免疫保护性研究

目的 探讨Th1和Th2类细胞因子对小鼠感染血吸虫的免疫保护作用. 方法 C57BL/6小鼠48只,抽签法随机均分为4组,白细胞介素（interleukin,IL）-4组,给予IL-4 （200 ng）; IL-12组,给予IL-12 （200 ng）;胸腺基质淋巴生成素（thymic stromal lymphopoietin,TSLP）组,给予TSLP（200 ng）;生理盐水组,给予生理盐水（200μl）.4组均为腹腔注射,每周3次,持续注射8周.给药后2周,各组小鼠经皮攻击感染日本血吸虫尾蚴（40±2）条/鼠,感染后第3、6周剖杀,计算各组小鼠虫荷数、肝脏卵荷数.芯片检测各组小鼠感染前和感染后不同时间点血清中IL-5、IL-10、γ-干扰素（interferon-γ,IFN-γ）及肿瘤坏死因子-α（tumor necrosis factor-α,TNF-α）的动态变化.观察小鼠肝、脾的病理变化. 结果 细胞因子注射的第8周,IL-4组的Th2类细因子IL-5、IL-10依次为161.97、65.47 μg/ml; TSLP组的IL-5、IL-10依次为132.72、77.18 μg/ml; IL-12组的IL-5、IL-10依次为87.15、12.29 μg/ml;生理盐水组的IL-5、IL-10依次为188.92、167.52 μg/ml.IL-4和TSLP组Th2类细胞因子水平均高于IL-12组,但低于生理盐水组.IL-12组IFN-γ、TNF-α依次为165.7、23.64 μg/ml,水平高于其它各组.感染后3周IL-4、IL-12、TSLP、生理盐水组虫荷数依次为（9.50±4.51）、（12.83±2.32）、（6.75±2.87）、（9.80±3.03）条;感染后6周上述各组虫荷数依次为（18.83±5.91）、（24.80±9.42）、（21.67±3.67）、（17.67±6.74）条,每克肝组织虫卵数依次为（58 286.98±25 351.60）、（80 460.18±35 542.66）、（54 579.56±16 399.21）、（41 094.92±25 598.27）.与生理盐水组相比,各实验组虫荷数与每克肝组织虫卵数差异均无统计学意义.感染后第3周,IL-4、IL-12、TSLP、生理盐水组脾指数依次为（0.010±0.002）、（0.011±0.002）、（0.009±0.001）、（0.007 ±0.001）,各实验组与生理盐水组相比,差异均有统计学意义（t=3.158、5.076、6.204,P＜0.05）.感染后第6?

The study on immunological protection induced by Th1 and Th2 cytokines in mice against Schistosoma japonicum

Objective To explore the immunologically protective effects of Th1 and Th2 cytokines in mice against Schistosoma japonicum infection.Methods 48 C57BL/6 mice were divided into four groups randomly,including interleukin （IL）-4,IL-12,thymic stromal lymphopoietin （TSLP） and saline group.In each group,each mouse was given cytokine intraperitoneally three times per week for 8 weeks continuously.The dosages were IL-4 group 200 ng each mouse,IL-12 group 200 ng each mouse,TSLP group 200 ng each mouse,and normal saline group 200 μl each mouse.Two weeks after administration,each mouse was infected with 40±2 cercariae percutaneously.Three and six weeks after infection,mice were sacrificed,and the number of worms and eggs in liver were calculated.Dynamic changes of IL-5,IL-10,interferon-γ （IFN-γ） and tumor necrosis factor-α （TNF-α） in serum of mice in different groups were detected by suspension array technology.Pathological changes of live and spleen in the mice were detected.Results Eight weeks after intraperitoneal injections of cytokines,levels of Th2 cytokines IL-5 and IL-10 in mice of IL-4 group were 161.97 and 65.47 μg/ml,which of TSLP group were 132.72 and 77.18 μg/ml,which of IL-12 group were 87.15 and 12.29 μg/ml,which of saline group were 188.92 and 167.52 μg/ml.Levels of Th2 cytokines in mice of IL-4 and TSLP groups were higher than that of IL-12 group,but lower than that of normal saline group.Levels of IFN-γ and TNF-α in the mice of IL-12 group were 165.7 and 23.64 μg/ml,which were higher than that of other groups.The worm burden of IL-4,IL-12,TSLP and saline group were（9.50±4.51）,（12.83±2.32）,（6.75±2.87）,（9.80±3.03）respectively three weeks after infection.The worm burdens of each group were （18.83±5.91）,（24.80±9.42）,（21.67±3.67）,（17.67±6.74）,and eggs per gram of liver were（58 286.98±25 351.60）,（80 460.18±35 542.66）,（54 579.56±16 399.21）,（41 094.92±25 598.27） respectively six weeks after infection.There were no significant d