枇杷花水提物防治小鼠急性酒精性肝损伤研究

目的初步观察枇杷花水提物（AELF）对急性酒精染毒小鼠的防治效果。方法取雄性昆明种小鼠，随机分为空白组、模型组、联苯双酯阳性药组、不同剂量AELF（400、800、1600mg·kg^-1）组，每组10只。按0．10ml／10g剂量灌胃给药10d，末次给药2h后一次性灌胃给予50％（Ⅳy）无水乙醇溶液0．12ml／10g，禁食不禁水16h。测定外周血象，血清中ALT、AST、ADH活性及肝匀浆中MDA、TG含量。结果与模型组相比，AELF各剂量组能明显降低WBC、AST、血清及肝脏TG水平，均以中剂量效果最好（P〈0．01，P〈0．01，P〈0．01，P〈0．05）；高剂量组能够明显降低血清ALT及肝脏MDA水平（P〈0．01，P〈0．05）。结论AELF可以通过降低染毒小鼠转氨酶和TG水平，提高机体抗氧化能力，对小鼠急性酒精性肝损伤起到一定的预防性保护作用。

Protective Effect of Aqueous Extract of Loquat Flower on Acute Alcohol-induced Liver Injury in Mice

Objective To investigate the effect of the aqueous extract of loquat flower （AELF） on acute alcoholic liver injury in mice. Methods The mice were randomly divided into 6 groups：control group, model group, positive drug group （Bifendate） ,and AELF groups at different doses （400,800,1600 mg·kg^-1）. Each group has 10 mice. Drugs were given once a day for 10 consecutive days,0.10 ml/10 g a time. 50% （ V/V ） dehydrated alco-hol of 0.12 ml/10 g was fed orally 2 h after the last administration except in control group. Then the animals were fasted for 16 h with water drinking allowed. Results Compared with model group, the AELF group at the dose of 800 mg ·kg^-1 underwent a marked decrease in the level of aspartate amino transferase （AST）, triglyceride （TG） of the serum and liver（ P 〈0.01, P 〈0.01, P 〈0.05） and the amount of WBC returned to normal（ P 〈0.01）, while in the 1600 mg·kg^-1 group the level of alanine amino transferase （ALT） in serum and the concentration of malondialdehyde （MDA） in liver homogenates was reduced significantly （ P 〈 0.01, P 〈 0.05 ）. Conclusion AELF can lower the level of ALT, AST, TG and improve the anti-oxidation ability of organisms. It has proactively protective effect on acute alcohol-induced liver injury in mice.