| HPLC法测定甲硝唑药膜的含量 |
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| 引用本文： | 刘眷光,;赵尚清,;李瑞林,;狄亚敏. HPLC法测定甲硝唑药膜的含量[J]. 解放军药学学报, 2014, 0(3): 251-252 |
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| 作者姓名： | 刘眷光, 赵尚清, 李瑞林, 狄亚敏 |
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| 作者单位： | [1]武警北京总队医院药房,北京100027; [2]总后勤部卫生部药品仪器检验所,北京100166 |
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| 摘 要： | 目的建立HPLC法测定甲硝唑药膜中甲硝唑的含量。方法以十八烷基硅烷键合硅胶为填充剂，甲醇-水（20：80）为流动相，检测波长为320nm；流速：1．0ml·min-1；柱温：30℃。结果甲硝唑在0．04998—0．59976mg·ml-1的范围内线性关系良好（r=0．9998），平均回收率为100．02％，RSD为0．50％（n=9）。结论本方法简便、测定结果准确，可用于甲硝唑药膜中甲硝唑的含量测定。
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| 关 键 词： | 甲硝唑药膜 甲硝唑 HPLC 含量测定 |
Determination of Metronidazole in Metronidazole Pellicles by HPLC |
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| Affiliation: | LIU Chun-guang, ZHAO Shang-qing, LI Rui-Lin, DI Ya-min (1.Beijing Corps Hospital,Chinese People＇s Armed Police Forces,Beijing 100027,China; 2.Institute for Drug and Instrument Control of Health Dept,GLD of PLA,Beijing 100166,China) |
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| Abstract: | Objective To establish an HPLC method for the content determination of metronidazole in met- ronidazole pellicles. Methods CAPCELL PAK Cls UG120 （4.6 mm× 250 mm ） was used as the chromatographic column, and the mobile phase was composed of methanol-water （20： 80）. The flow rate was 1.0 ml ·min-1 at a de- tection wavelength of 320 nm. The column temperature was 30 ℃. Results The calibration curves of metronidazole showed a good linear relationship over the range of 0. 049 98 -0. 599 76 mg ·ml-l（ r = 0. 9998）. The average re- covery of metronidazole was 100.02% and RSD was 0.50% （ n = 9）. Conclusion This method is simple and accu- rate. It can be used for the content determination of metronidazole in metronidazole pellicles. |
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| Keywords: | metronidazole pellicles metronidazole HPLC content determination |
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