| 人羊膜间充质干细胞在大鼠受损肝组织中分化为肝细胞**◆ |
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| 引用本文： | 宫黎明,方宁,陈代雄,万卫红,章涛,刘祖林,余丽梅,赵春华.人羊膜间充质干细胞在大鼠受损肝组织中分化为肝细胞**◆[J].中国神经再生研究,2011,15(36):6709-6713. |
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| 作者姓名： | 宫黎明 方宁 陈代雄 万卫红 章涛 刘祖林 余丽梅 赵春华 |
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| 作者单位： | 遵义医学院附属医院贵州省细胞工程重点实验室，贵州省遵义市563003,遵义医学院附属医院贵州省细胞工程重点实验室，贵州省遵义市563003,遵义医学院附属医院贵州省细胞工程重点实验室，贵州省遵义市563003,遵义医学院附属医院贵州省细胞工程重点实验室，贵州省遵义市563003,遵义医学院附属医院贵州省细胞工程重点实验室，贵州省遵义市563003,遵义医学院附属医院贵州省细胞工程重点实验室，贵州省遵义市563003,遵义医学院附属医院贵州省细胞工程重点实验室，贵州省遵义市563003,中国医学科学院基础医学研究所组织工程研究中心，北京市 100005 |
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| 基金项目： | “重大新药创制”国家科技重大专项课题(2009ZX09503-025)；贵州省科技计划发展项目(黔科合计字2051号；黔科合SZ字3017号) |
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| 摘 要： | 背景：羊膜间充质干细胞在体外适当的诱导条件下可分化为肝细胞样细胞，而在受损肝脏原位是否可分化为肝细胞值得探讨。
目的：观察人羊膜间充质干细胞在大鼠肝损伤原位植活及分化情况。
方法：采用胰酶-胶原酶二酶消化法从羊膜组织中分离人羊膜间充质干细胞。采用腹腔注射D-氨基半乳糖建立大鼠肝损伤模型，随机分为2组，人羊膜间充质干细胞移植组造模后注射L-DMEM 悬浮的人羊膜间充质干细胞悬液，对照组注射等量L-DMEM。
结果与结论：①FCM分析结果显示，所分离的人羊膜间充质干细胞表达CD29、CD44和CD166；免疫荧光染色显示人羊膜间充质干细胞表达波形蛋白，不表达CK19。②与对照组比较，人羊膜间充质干细胞移植后肝病理无明显差异，均呈急性肝坏死病理改变。③免疫荧光双染色结果显示，人羊膜间充质干细胞移植后1周主要定植于肝小叶且表达CK19，至2周表达CK18，至3周表达Alb。结果表明，人羊膜间充质干细胞在大鼠受损肝组织中能被植活，且可分化为肝细胞，提示人羊膜间充质干细胞移植在临床肝病的治疗方面可能具有潜在应用价值。
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| 关 键 词： | 人羊膜间充质干细胞 移植 分化 肝细胞 肝损伤 |
| 收稿时间： | 3/1/2011 12:00:00 AM |
Differentiation of human amnion derived-mesenchymal stem cells into hepatocytes in rat injured liver |
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| Gong Li-ming,Fang Ning,Chen Dai-xiong,Wan Wei-hong,Zhang Tao,Liu Zu-lin,Yu Li-mei and Zhao Chun-hua.Differentiation of human amnion derived-mesenchymal stem cells into hepatocytes in rat injured liver[J].Neural Regeneration Research,2011,15(36):6709-6713. |
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| Authors: | Gong Li-ming Fang Ning Chen Dai-xiong Wan Wei-hong Zhang Tao Liu Zu-lin Yu Li-mei and Zhao Chun-hua |
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| Institution: | Key Laboratory of Cell Engineering of Guizhou Province, Affiliated Hospital of Zunyi Medical College, Zunyi 563003, Guizhou Province, China,Key Laboratory of Cell Engineering of Guizhou Province, Affiliated Hospital of Zunyi Medical College, Zunyi 563003, Guizhou Province, China,Key Laboratory of Cell Engineering of Guizhou Province, Affiliated Hospital of Zunyi Medical College, Zunyi 563003, Guizhou Province, China,Key Laboratory of Cell Engineering of Guizhou Province, Affiliated Hospital of Zunyi Medical College, Zunyi 563003, Guizhou Province, China,Key Laboratory of Cell Engineering of Guizhou Province, Affiliated Hospital of Zunyi Medical College, Zunyi 563003, Guizhou Province, China,Key Laboratory of Cell Engineering of Guizhou Province, Affiliated Hospital of Zunyi Medical College, Zunyi 563003, Guizhou Province, China,Key Laboratory of Cell Engineering of Guizhou Province, Affiliated Hospital of Zunyi Medical College, Zunyi 563003, Guizhou Province, China,Tissue Engineering Center, Institute of Basic Medical Sciences, Chinese Academy of Medical Sciences, Beijing 100005, China |
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| Abstract: | |
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| Keywords: | human amnion derived-mesenchymal stem cell transplantation differentiation hepatocyte liver injury |
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