镓盐对骨质疏松症大鼠骨组织中羟基磷灰石及胶原含量的影响

背景：硝酸镓是近10多年来发现的一种能降低骨转换的药物。目的：观察硝酸镓对骨质疏松大鼠骨羟基磷灰石及胶原含量的影响。方法：实验分为3组。正常组大鼠开腹切除1小块脂肪,其余大鼠切除双侧卵巢制作大鼠骨质疏松症动物模型。正常组及骨质疏松组腹腔注射生理盐水,硝酸镓组腹腔注射硝酸镓,3次/周,常规饲料喂养12周后取大鼠双侧股骨。结果与结论：骨质疏松组大鼠骨组织中羟基磷灰石含量明显高于正常组(P < 0.05);硝酸镓组与正常组相比有升高趋势(P > 0.05),与骨质疏松组间差异无显著性意义(P > 0.05),骨质疏松组骨中胶原较正常组及硝酸镓组明显降低(P < 0.05)。结果表明,发生骨质疏松时骨中胶原含量明显降低,羟基磷灰石含量有升高趋势,硝酸镓可以抑制这一过程。

Effects of gallium nitrate on the content of hydroxyapatite and collagen in osseous tissues of rats with osteoporosis

BACKGROUND: In recent years, it is discovered that gallium nitrate can decrease bone conversion. OBJECTIVE: To observe the effects of gallium nitrate on the content of hydroxyapatite and collagen in rats with osteoporosis.METHODS: The rats were divided into control group, osteoporosis group and gallium nitrate group. Osteoporosis rat models were made by cutting off the bilateral ovaries in the latter two groups. The rats in the gallium nitrate therapy group were treated with 1 mg/kg gallium nitrate, three times a week through abdominal cavity; those in the control and osteoporosis groups were administrated with normal saline. The rats in the three groups had free access to water and standard food. All the contents of hydroxypatite and collagen in osseous tissue were determined and analyzed. RESULTS AND CONCLUSION: The content of hydroxyaptite between the osteoporosis group and normal control group was different significantly (P < 0.05); there were no significant differences among gallium nitrate therapy group, control group and osteoporosis group (P > 0.05), but the content of hydroxyaptite in the gallium nitrate group had increasing trend compared with that in the normal group. The level of collagen in osteoporosis group was obviously lower than that in normal control group and gallium nitrate therapy group (P < 0.05). When osteoporosis happened, the level of collagen in osteoporosis rats decreased and the content of hydroxyapatite was in the trend of increasing, and gallium nitrate could inhibit the process.