5-氮杂胞苷诱导人脐带间充质干细胞向心肌样细胞的分化☆

背景：5-氮杂胞苷能诱导人脐带间充质干细胞分化为心肌细胞。目的：以5-氮杂胞苷诱导人脐带间充质干细胞分化为心肌细胞。方法：采用贴壁培养法分离、纯化人脐带间充质干细胞，以5-氮杂胞苷诱导第3代人脐带间充质干细胞分化为心肌样细胞。结果与结论：诱导前人脐带间充质干细胞呈典型的梭形；诱导后一二周细胞体积变大，三四周后相邻细胞间胞膜有接触，逐渐相连呈肌管状，细胞胞质内可见细丝样结构。诱导4周后免疫组织化学鉴定人脐带间充质干细胞cTnI表达阳性，未诱导细胞cTnI表达阴性；诱导组Nkx2.5、GATA 4 mRNA表达水平较未诱导组显著增加(P < 0.05)。提示5-氮杂胞苷可能通过调控GATA4、Nkx2.5基因的表达促进人脐带间充质干细胞分化为心肌样细胞，并促进其成熟。

5-azacytidine induces the differentiation of human umbilical cord derived mesenchymal stem cells into cardiomyocytes

BACKGROUND: 5-azacytidine (5-aza) can induce the differentiation of human umbilical cord derived mesenchymal stem cells (hUCMSCs) into cardiomyocytes.OBJECTIVE: To investigate the cellular mechanism underlying the differentiation of hUCMSCs into cardiomyocytes induces by 5-aza. METHODS: hUCMSCs were isolated and purified by adherent method. hUCMSCs at passage 3 were induced to differentiate into cardiomyocytes by 5-aza. RESULTS AND CONCLUSION: Spindle-shaped hUCMSCs were presented from human umbilical cord tissue by adherent culture method. hUCMSCs treated with 5-aza had long cytoplasmic process 1-2 weeks after induction, and they were connected with adjacent cells forming myotube-like structures 3-4 weeks after induction. hUCMSCs treated with 5-aza were stained positively for cTnI 4 weeks after induction. Statistically higher level expression of Nkx2.5 and GATA 4 mRNA in induced group was observed in comparison with those of control group (P < 0.05). These indicated that hUCMSCs can be induced to differentiate into cardiomyocytes by 5-aza in vitro through up-regulating the expression of Nkx2.5 and GATA4.