两种血细胞分离机干/祖细胞采集和移植效果的比较

背景：外周血造血干/祖细胞通过血细胞分离机进行体外收集,血细胞分离机的性能、工作状况将直接关系到采集物的特性和数量,从而直接影响受者的造血重建。目的：比较Fenwal CS-3000 Plus与Amicus两种血细胞分离机采集外周血造血干/祖细胞及受者移植效果。方法：共入选接受异基因外周血干细胞移植患者51例,Fenwal CS-3000 Plus组27例,平均年龄(34.2±10.6)岁;Amicus组24例,平均年龄(35.4±12.1)岁。比较两组采集物有核细胞数、CD34+细胞数、采集效率、红细胞与血小板采集量及造血重建时间。结果与结论：两组采集物的有核细胞数、CD34+细胞数、采集效率、红细胞采集量及造血重建时间差异均无显著性意义;Fenwal CS-3000Plus组血小板采集量明显高于Amicus组(P < 0.01)。结果显示Fenwal CS-3000 Plus与Amicus血细胞分离机在分离外周血干/祖细胞方面和移植效果没有差异;血小板较低被采集者使用Amicus血细胞分离机采集可能更为安全。

Comparison of two blood cell separators in collection of peripheral blood stem cell/progenitor cells and effectiveness of transplantation

BACKGROUND: Peripheral blood stem cells/progenitor cells were collected in vitro using blood cell separators, which directly affects the features and quantity of collected cells, additionally, affects hematopoietic reconstitution of recipients. OBJECTIVE: To evaluate the efficiencies of Fenwal CS-3000 Plus and Amicus blood cell separators in collecting peripheral blood stem cells/progenitor cells and effectiveness of transplantation. METHODS: Totally 51 cases received allogenic peripheral blood stem cells transplantation were selected, including 27 cases, mean aged (34.2±10.6) years, who performed stem cell apheresis procedures using Fenwal CS-3000 Plus blood cell separator and 24 donors, mean aged (35.4±12.1) years, using Amicus blood cell separator. The number of nucleated cells and CD34+ cells, collection efficiency, collection amount of red blood cell and the time of the hematopoieteic reconstitution of two devices were compared. RESULTS AND CONCLUSION: There were no significant differences in the total number of nucleated cells and CD34+ cells, collection efficiency, collection amount of red blood cell and the time of the hematopoieteic reconstitution between the two devices. However, the amount of platelet in the stem/progenitor cell products of the Fenwal CS-3000Plus group was obviously greater than that of the Amicus group (P < 0.01). There is no difference in the collection efficiencies of the two blood cell separators in the peripheral blood stem cells/progenitor cell and the effectiveness of transplantation. The Amicus cell separator is better for the donors with thrombocytopenia.