丹参对大鼠移植肝血红素氧合酶1表达的影响*

背景：器官移植前使用丹参预处理能够保护组织缺血-再灌注损伤，改善移植器官存活率。目的：观察含丹参的冷灌注液对同种异体大鼠移植肝脏中血红素氧合酶1表达的影响，以及对供体肝脏缺血-再灌注损伤的保护作用。方法：将SD雄性大鼠随机分成UW液组(术中使用UW液灌注保存)、丹参+UW液组(术中使用丹参+UW液灌注保存)、ZnPP预处理组(移植前24 h腹腔内注射ZnPP，术中使用丹参+UW液灌注保存)，建立稳定的大鼠同种异体肝移植模型。同时取10只正常大鼠作为正常对照。结果与结论：丹参+UW液组和UW液组血清总胆红素、谷丙转氨酶、谷草转氨酶水平明显低于ZnPP预处理组(P < 0.01)。血红素氧合酶1mRNA及其蛋白在丹参+UW液组中较UW组表达更明显，在ZnPP预处理组中表达明显受到抑制(P < 0.05)。丹参+UW液组肝脏Suzuki标准评分明显低于ZnPP预处理组及UW液组(P < 0.05)。表明丹参能上调同种异体的大鼠移植肝脏中血红素氧合酶1 mRNA及其蛋白的表达，减轻供肝缺血-再灌注损伤，保护移植大鼠肝脏。

Effects of Danshen on heme oxygenase-1 expression in rat transplanted liver

BACKGROUND: Danshen pretreatment before organ transplantation can protect tissue from ischemia-reperfusion injury and improve the survival rate of graftsOBJECTIVE: To investigate the effects of Danshen-containing cold perfusion solution on heme oxygenase-1 (HO-1) expression in rat liver allograft and the protective effects on donor liver from ischemia-reperfusion injury.METHODS: Sprague-Dawley male rats were randomly divided into University of Wisconsin solution (UW) solution group (UW group, preservation using UW solution during transplantation), Danshen+UW solution group (DS+UW group, preservation with Danshen+UW solution during transplantation), ZnPP (HO-1 inhibitor) pretreatment group (ZnPP group, intraperitoneal injection of ZnPP 24 hours before transplantation, and preservation with Danshen+UW solution during transplantation). Reliable rat models of liver allografting were established. Ten normal rats were selected as normal controls. RESULTS AND CONCLUSION: Serum total bilirubin, alanine aminotransferase, and aspartate aminotransferase levels were significantly lower in the DS+UW group, UW group than in the ZnPP group (P < 0.01). HO-1 mRNA and protein expressions were greater in the DS+UW group than in the UW group, but the expressions were obviously inhibited in the ZnPP group (P < 0.05). Suzuki scores were significantly lower in the DS+UW group than in the ZnPP group and UW group. These findings suggest that Danshen can upregulate HO-1 mRNA and protein expressions in rat liver allograft, alleviate ischemia-reperfusion injury of donor liver, and protect the transplanted liver.