心肌营养素1对谷氨酸诱导神经干细胞凋亡的作用**

背景：心肌营养素1属于白细胞介素6细胞因子家族，近年发现其可能对中枢神经系统有重要保护作用。 目的：探索重组腺病毒心肌营养素1对谷氨酸诱导神经干细胞凋亡的保护作用，为脑损伤提供新的治疗措施。 方法：取新生Wistar大鼠海马神经干细胞，第2代培养第5天用于实验，神经干细胞分为3组：谷氨酸组、重组腺病毒心肌营养素1组及对照组，利用四唑蓝比色了解细胞存活情况及原位缺口末端标记技术检测细胞凋亡。 结果与结论：谷氨酸组神经干细胞呈现明显形态改变，重组腺病毒心肌营养素1组未见类似改变，在谷氨酸暴露后12，24，48及72 h MTT吸光度值均低于心肌营养素1组，而细胞凋亡率均高于心肌营养素1组。与其他两组比较，对照组相同时间点A值增高，细胞凋亡率降低。提示，心肌营养素1对谷氨酸损伤的神经干细胞具有保护作用，可以抑制神经干细胞凋亡，促进其存活和增殖。

Effects of cardiotrophin-1 on glutamate induced apoptosis of neural stem cells

BACKGROUND: Cardiotrophin-1 (CT-1) appertains to interleukin-6 cytokine family, which is found in recent years to have an important protective role in the central nervous system. OBJECTIVE: To observe the protective effects of CT-1 on the apoptosis of neural stem cells (NSCs) induced by glutamate. METHODS: Hippocampal neural stem cells isolated from newborn Wistar rats were passaged and divided into 3 groups: glutamate, CT-1 (transfected with Adv CT-1) and control groups. NSCs survivals were estimated by MTT colorimetric method, and the apoptosis of NSCs was detected by means of TdT-Mediated dUTP nick end labeling (TUNEL). RESULTS AND CONCLUSION: Glutamate treatment alone induced cell death that was associated with obvious morphological changes, while these changes were inhibited by transfection of CT-1 into NSCs. At 12, 24, 48 and 72 hours after treatment with glutamate, the absorbance values of MTT in the lutamate group were significantly lower than those in the CT-1 group, the apoptosis rates of NSCs in the glutamate group were significantly higher than those in the CT-1 group. At the same time, the absorbance values of MTT in the control group were significantly higher than those in the glutamate group and CT-1 group, the apoptosis rates of NSCs were significant lower than those in the glutamate group and CT-1 group. These indicated that CT-1 has a protective effect on NSCs injured by glutamate, can inhibit NSCs apoptosis and improve NSCs survival.