表皮生长因子干预人羊膜间充质干细胞的增殖和迁移

背景：人羊膜间充质干细胞移植可促进皮肤创伤的愈合。 目的：观察表皮生长因子对体外培养人羊膜间充质干细胞增殖和迁移的影响。 方法：人羊膜间充质干细胞经分离、鉴定并培养至第3代。细胞经过不同浓度的表皮生长因子处理后，用MTT法测量吸光度值从而计算出细胞存活率，通过计数穿过Transwell小室的细胞数测定表皮生长因子对细胞迁移能力的影响；并用表皮生长因子受体的抑制剂AG1478及PI3K的抑制剂LY294002检测其对细胞迁移能力的影响。 结果与结论：表皮生长因子处理后细胞的吸光度值升高1.55倍，穿过膜的数量增高1.5倍；AG1478、LY294002对表皮生长因子引起的迁移有抑制作用。提示表皮生长因子处理后细胞增殖和迁移能力均增强，迁移与EGFR-PI3K信号通路有关。

Effect of epidermal growth factor on proliferation and migration of human amniotic mesenchymal stem cells

BACKGROUND: Human amniotic mesenchymal stem cells (hAMSCs) have the ability to improve skin wound healing. OBJECTIVE: To explore the effects of epidermal growth factor (EGF) on hAMSCs proliferation and migration in vitro. METHODS: Through isolation, identification and culture, the third generation of hAMSCs were obtained. After treatment with different concentrations of EGF, the absorbance was measure to calculate survival rate of the cells using MTT method. We also counted the number of cells through Transwell to determine the effect of EGF on cell migration. With inhibitors AG1478, LY294002 of EGFR and PI3K, we tested their influence on cell migration. RESULTS AND CONCLUSION: EGF caused 1.55 times increase in the absorbance, and 1.5 times increase in the number of cell across the film. So AG1478 and LY294002 had inhibitory effect on the migration hAMSCs treated with EGF. It is indicated that the proliferation and migration ability of hAMSCs enhanced following treatment with EGF, and hAMSCs migration is related to PI3K signaling pathways.