去卵巢牙周炎模型大鼠牙槽骨吸收与补骨脂的干预

背景：补骨脂在治疗骨质疏松症方面有明显效果。 目的：观察补骨脂对去卵巢牙周炎大鼠牙槽骨代谢、牙槽骨密度及高度的影响。 方法：将30只雌性Wistar大鼠随机等分为3组：去卵巢组和补骨脂组行双侧卵巢切除术，假手术组仅手术不切除卵巢，补骨脂组于卵巢切除第2天灌胃3 g/kg补骨脂水剂，1次/d。去卵巢2周，所有大鼠采用结扎上颌磨牙的方法建立牙周炎模型。 结果与结论：上颌磨牙结扎12周，与假手术组比较，去卵巢组大鼠血清雌二醇、钙离子浓度显著降低(P < 0.05)，碱性磷酸酶水平显著升高(P < 0.05)，X射线片及苏木精-伊红染色显示去卵巢组大鼠上颌骨骨密度及高度显著降低(P < 0.05)；与去卵巢组比较，补骨脂组大鼠血清钙离子浓度显著升高(P < 0.05)，碱性磷酸酶水平显著降低(P < 0.05)，上颌骨骨密度及高度显著增高(P < 0.05)，且与假手术组比较差异无显著性意义(P > 0.05)。说明雌激素缺乏促进实验性牙周炎大鼠牙槽骨吸收，补骨脂可有效阻止雌激素降低导致的牙槽骨高度、骨密度降低。

Effects of psoralea corylifolia on alveolar bone absorption in an ovariectimized rat model of periodontitis

BACKGROUND: Psoralea corylifolia exhibits obvious therapeutic effects on osteoporosis. OBJECTIVE: To investigate the effects of psoralea corylifolia on alveolar bone metabolism, alveolar bone mineral density and height in ovariectimized rats with periodontitis. METHODS: Thirty female Wister rats were randomly divided into three groups: ovariectomy, psoralea corylifolia and sham-surgery. Rats from the ovariectomy and psoralea corylifolia groups underwent bilateral ovariectomy, and rats from the sham surgery group did not receive ovariectomy. At 2 days after ovariectomy, rats from the psoralea corylifolia group were orally administered 3 g/kg psoralea corylifolia, once a day. At 2 weeks after ovariectomy, all rats were established into peridontitis models by ligation of maxillary molar. RESULTS AND CONCLUSION: At 12 weeks after ligation of maxillary molar, compared with sham surgery group, estradiol and calcium ion levels were significantly decreased (P < 0.05), alkaline phosphatase level was significantly increased (P < 0.05) in the ovariectomy group. X-ray and hematoxylin-eosin staining showed that alveolar bone mineral density and height in the ovariectomy group was significantly decreased than that in the control group (P < 0.05). Compared with ovariectomy group, serum level of calcium ion was significantly increased (P < 0.05) and alkaline phosphatase level was significantly decreased (P < 0.05), and alveolar bone mineral density and height were significantly increased in the psoralea corylifolia group. However, no significant difference was observed between psoralea corylifolia group and sham surgery group (P > 0.05). These findings suggest that estrogen-deficient state can promote alveolar bone absorption in rats with experimental periodontitis, and psoralea corylifolia can prevent the decrease in alveolar bone height and density resulting from estrogen deficiency.