广西百色地区壮族男性人群骨密度与脂联素基因单核苷酸多态性的关系***

背景：脂联素可在骨代谢中发挥重要作用。目的：观察脂联素基因单核苷酸多态性与广西百色地区壮族男性骨密度的关系。方法：选取广西百色地区壮族男性跟骨骨量减少患者，采用单碱基延伸的单核苷酸多态性分型技术对广西百色地区302例壮族男性的脂联素基因的5个单核苷酸多态性位点(rs1063539、rs12495941、rs266729和rs3774261)进行了基因分型。结果与结论：以5个多态性位点作为自变量的多元 Logistic回归检测结果显示，仅rs3774261多态性与跟骨超声振幅衰减显著相关(OR=1.948，95%CI：1.184~3.203，P < 0.01)，并独立于骨量减少的传统危险因素。对基因型进行纯合子与杂合子合并后的协方差分析显示，仅rs3774261的AG+GG与AA基因型的跟骨超声振幅衰减值差异有显著性意义(P < 0.05)，AG+GG型对骨密度具有一定的保护作用，AA型是骨密度降低的危险因素。结果证实，脂联素基因第2内含子rs3774261位点多态性与中国百色地区壮族男性骨密度有一定关联。

Association between adiponectin gene single nucleotide polymorphisms and bone mineral density in a male population of Zhuang nationality in Guangxi Baise area

BACKGROUND: Studies have shown that adiponectin plays an important role in bone metabolism, but the relationship between adiponectin gene polymorphism and bone metabolism in osteoporosis has rarely been reported.OBJECTIVE: To investigate the correlation between adiponectin gene 5 single nucleotide polymorphisms and bone mineral density in Guangxi Zhuang nationality men. METHODS: A case-control study was carried out on osteopenia patients. Genotypes for adiponectin gene 5 loci (rs1063539, rs12495941, rs266729, rs3774261 and rs710445) polymorphism in 302 Guangxi Zhuang nationality men were determined by Multiplex SNaPshot. RESULTS AND CONCLUSION: rs1063539, rs12495941, rs266729 and rs710445 polymorphisms were met with Hardy-Weinberg equilibrium (P > 0.05). Five loci genotype frequencies in the normal group and the osteopenia group met with Hardy-Weinberg equilibrium (P > 0.05). There was no significant difference in the genotype distributions of five loci polymorphism between low bone mass group and control group (P > 0.05). After adjustments for age, weight, height, movement and body mass index, multivariate Logistic regression analyses revealed that only rs3774261 polymorphism remained significantly associated with low bone mass (P < 0.01). The subjects with the combined AA genotype had higher risk of low bone mass compared with those with the AG+GG genotype (adjusted OR =1.948, 95%CI: 1.184-3.203, P=0.009). Polymorphism of rs3774261 was independently correlated with calcaneal low bone mass in Baise Zhuang nationality male population. The results confirm that, adiponectin gene intron 2 rs3774261 polymorphism and bone mineral density in Baise Zhuang nationality men have some relevance. The presence of the AA genotype may dominantly increase the risk of osteopenia. AG+GG genotype has a protective effect on bone mineral density. The data also suggest that the rs1063539, rs12495941, rs266729 and rs710445 polymorphism might not be associated with osteopenia in Baise Zhuang nationality men.