| 真核表达载体p4CCL20-ZsGreen1-DR的构建与鉴定 |
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| 引用本文： | 王勇,王志中,钟兵,王恒,邹庆华,陈渝.真核表达载体p4CCL20-ZsGreen1-DR的构建与鉴定[J].中国神经再生研究,2011,15(41):7719-7722. |
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| 作者姓名： | 王勇 王志中 钟兵 王恒 邹庆华 陈渝 |
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| 作者单位： | 解放军第三军医大学西南医院，风湿科，重庆市 400038,解放军第三军医大学西南医院，风湿科，重庆市 400038,解放军第三军医大学西南医院，风湿科，重庆市 400038,解放军第三军医大学西南医院，风湿科，重庆市 400038,解放军第三军医大学西南医院，风湿科，重庆市 400038,解放军第三军医大学西南医院，烧伤研究所，创伤、烧伤、复合伤国家重点实验室，重庆市 400038 |
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| 基金项目： | 重庆市科委自然基金面上项目(2008BB5133)；国家自然基金面上项目(30973827) |
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| 摘 要： | 背景：抗炎药物高通量筛选体系的建立，可为相关药物的研究提供一个理想的技术平台。
目的：构建以核因子κB顺式作用元件4×CCL20基序为增强子，以SV40为启动子，以ZsGreen1-DR为报告基因的真核表达载体p4CCL20-ZsGreen1-DR。
方法：以PGL2-control质粒为模板，PCR扩增目的片段SV40，两侧引入KpnⅠ/BamHⅠ酶切位点，克隆至pZsGreen1-DR质粒的KpnⅠ/BamHⅠ酶切位点中，构建成pSV40-ZsGreen1-DR载体。将4×CCL20基序双链DNA克隆到pSV40-ZsGreen1- DR载体的BglⅡ和EcoRⅠ酶切位点之间，构建p4CCL20-ZsGreen1-DR重组质粒。
结果与结论：经过DNA测序分析证实p4CCL20-ZsGreen1-DR重组质粒构建成功。该重组质粒可作为抗炎药物高通量筛选体系的基础。
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| 关 键 词： | 载体构建 CC亚族趋化因子配体20 不稳定型绿色荧光蛋白 测序分析 |
| 收稿时间： | 3/7/2011 12:00:00 AM |
| 修稿时间： | 3/7/2011 12:00:00 AM |
Construction and identification of p4CCL20-ZsGreen1-DR eukaryotic expression vector |
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| Wang Yong,Wang Zhi-zhong,Zhong Bing,Wang Heng,Zou Qing-hua and Chen Yu.Construction and identification of p4CCL20-ZsGreen1-DR eukaryotic expression vector[J].Neural Regeneration Research,2011,15(41):7719-7722. |
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| Authors: | Wang Yong Wang Zhi-zhong Zhong Bing Wang Heng Zou Qing-hua and Chen Yu |
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| Institution: | Department of Rheumatology State Key Laboratory of Trauma, Burns and Combined Injury, Southwest Hospital, Third Military Medical University of Chinese PLA, Chongqing 400038, China,Department of Rheumatology State Key Laboratory of Trauma, Burns and Combined Injury, Southwest Hospital, Third Military Medical University of Chinese PLA, Chongqing 400038, China,Department of Rheumatology State Key Laboratory of Trauma, Burns and Combined Injury, Southwest Hospital, Third Military Medical University of Chinese PLA, Chongqing 400038, China,Department of Rheumatology State Key Laboratory of Trauma, Burns and Combined Injury, Southwest Hospital, Third Military Medical University of Chinese PLA, Chongqing 400038, China,Department of Rheumatology State Key Laboratory of Trauma, Burns and Combined Injury, Southwest Hospital, Third Military Medical University of Chinese PLA, Chongqing 400038, China,Institute of Burn Research, State Key Laboratory of Trauma, Burns and Combined Injury, Southwest Hospital, Third Military Medical University of Chinese PLA, Chongqing 400038, China |
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| Abstract: | |
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| Keywords: | vector CC chemokine ligand 20 unstable green fluorescent protein sequencing analysis |
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