甲醛熏染模型大鼠肝脏组织增殖细胞核抗原表达与三七复方合剂的干预

背景：甲醛作为一种有毒物质可给人体带来严重的损害，同时又缺少有效的防治方法。 目的：观察中药三七复方合剂对甲醛熏染大鼠肝细胞增殖的影响。方法：将SD大鼠随机分成对照组、甲醛组和三七治疗组。对照组不做任何处理；甲醛组每天置于静态熏染箱内行甲醛熏染，连续8周；三七治疗组处理同甲醛组，但自第5周起，每日给予三七复方合剂灌胃1次。结果与结论：苏木精-伊红染色结果显示，甲醛熏染8周甲醛组大鼠肝组织肝小叶形态结构紊乱、肝细胞呈现明显的增殖现象，窦间隙缩窄，而三七治疗组肝组织的形态结构明显恢复。免疫组织化学结果显示，甲醛组大鼠肝组织增殖细胞核抗原阳性细胞数明显多于对照组(P < 0.01)，而三七治疗组与甲醛组相比则明显降低(P < 0.01)。结果证实，三七复方合剂能够降低由甲醛诱发的肝组织增殖细胞核抗原的表达，可有效减缓由甲醛诱发的大鼠肝细胞增殖。

Effects of compound panax notoginseng on proliferating cell nuclear antigen expression in liver tissue of rats exposed to formaldehyde vapor

BACKGROUND: Formaldehyde is a commonly used chemical substance which can cause serious damage to the body as a toxicant, and there is lack of effective control methods.OBJECTIVE: To explore the curative effect of compound panax notoginseng (CPN) on the proliferation of hepatic cells of Sprague-Dawley rats exposed to formaldehyde vapor. METHODS: Eighteen Sprague-Dawley male rats were randomly divided into three groups: control, formaldehyde and CPN. Nothing was done to the control group rats. The formaldehyde group rats were exposed to formaldehyde vapor in static toxification chambers, 8 hours/day, 5 days/week, for a total of 8 weeks. The CPN group rats were treated as the same the formaldehyde group, with exception of intragastric administration of CPN once a day since the 5th week. RESULTS AND CONCLUSION: Hematoxylin-eosin staining results showed that the hepatic lobule morphology of the formaldehyde group significantly disordered at the 8th week, which suggested an obvious proliferation state, and the hepatic sinusoid was narrower compared with the control group; however, the morphological structure of liver tissue in the CPN group was greatly recovered. Immunohistochemical results showed that the expression of proliferating cell nuclear antigen (PCNA) was significantly increased at the 8th week in the formaldehyde group than in the control group (P < 0.01), but the expression was significantly decreased in the CPN group than in the formaldehyde group (P < 0.01). These findings suggest that CPN can decrease the expression of PCNA which was induced by formaldehyde and effectively alleviate formaldehyde-induced hepatic cell proliferation, therefore exhibiting beneficial effects on the liver of SD rats exposed to formaldehyde vapor.