| 亚低温可促进脑出血大鼠内源性神经干细胞的增殖* |
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| 引用本文： | 张隽,祝平,侯丹慧,王连坤,王 莹,王昆祥.亚低温可促进脑出血大鼠内源性神经干细胞的增殖*[J].中国神经再生研究,2011,15(32):5941-5944. |
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| 作者姓名： | 张隽 祝平 侯丹慧 王连坤 王 莹 王昆祥 |
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| 作者单位： | 哈尔滨医科大学附属第四医院神经内科，黑龙江省哈尔滨市 150001；哈尔滨医科大学附属第一医院神经内科，黑龙江省哈尔滨市 150001,哈尔滨红十字医院急诊科，黑龙江省哈尔滨市 150000,哈尔滨医科大学附属第四医院神经内科，黑龙江省哈尔滨市 150001,黑龙江省医院神经内科，黑龙江省哈尔滨市 150001,哈尔滨医科大学附属第一医院神经内科，黑龙江省哈尔滨市 150001,佳木斯大学附属第一医院神经内科，黑龙江省佳木斯市 154001 |
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| 基金项目： | 黑龙江省教育厅科学技术研究项目(11541152) |
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| 摘 要： | 背景：亚低温对脑出血后脑组织保护作用的研究多集中在减轻脑水肿方面，对神经干细胞的增殖是否有促进作用研究很少。
目的：观察亚低温对脑出血大鼠血肿周围、侧脑室旁神经干细胞增殖的影响。
方法：采用自体血注入尾状核制作Wistar大鼠脑出血模型，亚低温组于制作模型后给予局部亚低温4 h，对照组给予常温处理。
结果与结论：脑出血后1，3，7，14 d，亚低温组Longa 5分制法评分低于对照组(P < 0.05)。免疫组织化学方法检测亚低温组各时间点血肿周边及侧脑室旁组织的BrdU阳性细胞数明显多于对照组(P < 0.05)。初步提示亚低温处理可以促进干细胞内源性增殖，对脑出血有保护作用。
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| 关 键 词： | 神经干细胞 亚低温 脑出血 BrdU 增殖 |
Neuroprotective effect of mild hypothermia on the proliferation of endogenous neural stem cells in rats with intracerebral hemorrhage |
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| Zhang Jun,Zhu Ping,Hou Dan-hui,Wang Lian-kun,Wang Ying and Wang Kun-xiang.Neuroprotective effect of mild hypothermia on the proliferation of endogenous neural stem cells in rats with intracerebral hemorrhage[J].Neural Regeneration Research,2011,15(32):5941-5944. |
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| Authors: | Zhang Jun Zhu Ping Hou Dan-hui Wang Lian-kun Wang Ying and Wang Kun-xiang |
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| Institution: | Department of Neurology, the Fourth Clinical Hospital of Harbin Medical University, Harbin 150001, Heilongjiang Province, China; Department of Neurology, the First Clinical Hospital of Harbin Medical University, Harbin 150001, Heilongjiang Province, China,Department of Emergency, Harbin Red Cross Central Hospital, Harbin 150000, Heilongjiang Province, China,Department of Neurology, the Fourth Clinical Hospital of Harbin Medical University, Harbin 150001, Heilongjiang Province, China,Department of Neurology, Heilongjiang Provincial Hospital, Harbin 150001, Heilongjiang Province, China,Department of Neurology, the First Clinical Hospital of Harbin Medical University, Harbin 150001, Heilongjiang Province, China,the First Clinical Hospital of Jiamusi Medical University, Jiamusi 154001, Heilongjiang Province, China |
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| Abstract: | BACKGROUND: There are a few reports addressing application of mild hypothermia to brain edema after intracerebral hemorrhage, but few reports have addressed mild hypothermia effects on proliferation of neural stem cells in the brain following intracerebral hemorrhage.
OBJECTIVE: To explore the effects of mild hypothermia on proliferation of neural stem cells of rats after intracerebral hemorrhage.
METHODS: A model of intracerebral hemorrhage was established by autologous arterial blood injected into right caudate nucleus. Local mild hypothermia was applied in the mild hypothermia group for 4 hours after the blood injected in the brain. Normal body temperature was maintained in control group.
RESULTS AND CONCLUSION: At 1, 3, 7, 14 days after intracerebral hemorrhage, the Longa score in the hypothermia group was lower than that in the control group (P < 0.05). At various time points following intracerebral hemorrhage, the number of BrdU-positive cells was obviously greater in the hypothermia group compared with the control group (P < 0.05). Results had indicated that mild hypothermia can promote the proliferation of neural stem cells, and shows neuroprotective effects on intracerebral hemorrhage. |
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| Keywords: | neural stem cell hypothermia intracerebral hemorrhage |
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