电针对大白鼠脊神经节内乙酰胆碱含量和乙酰胆碱酯酶活性的影响

<正> 脊神经节是体躯一级感觉神经元胞体集积区,是研究一级感觉传入神经化学性质的一个重要部位。我室曾用组织化学的方法发现脊神经节内含有乙酰胆碱酯酶(AchE),本文将以生化的方法研究电针和切断后根后脊神经节内Ach含量及脊神经节和脊髓后角内AchE活性的变化,旨在探讨外周乙酰胆碱(Ach)与针感一级传入的关系。

Influence of electroacupuncture on ACh content and AChE activity in the spinal ganglia of rat

The spinal ganglion a cell body storage of somatic primary-sensory neurons, is considered as an important region for neurochemical research of primary afferent nerves. The present experiment aims at the observation on the Ach content and its changes in the ganglia of 128 rats by means of biochemical methods under the effect of administration of Neostigmini and application of electroacupuncture. It is found that there are evidence of existence of Ach in the ganglia and when the AchE activity in the periphery nerve system is inhibited, the Ach content of ganglia rose. In addition, during acupuncture (applied at acupoint "Huantiao") analgesia, no apparent changes of Ach content can be visualized in spinal ganglia, however, the level of AchE activity goes up evidently. The foregoing results indicate that in the somatic primary-sensory neuron Ach is one of important neurotransmitters which may influence and take part in the transmission of sensory information through primary afferent nerves.