Light chain composition of CSF oligoclonal IgG bands in multiple sclerosis and subacute sclerosing penecephalitis |
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Authors: | David H. Mattson Raymond P. Roos John E. Hopper Barry G. W. Arnason |
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Affiliation: | 1. Department of Neurobiology, The University of Chicago, Pritzker School of Medicine, 950 E. 59th Street, Chicago, IL 60637, U.S.A.;2. Department of Medicine, University of Illinois Hospital, 840 S. Wood Street, Chicago, IL 60680, U.S.A. |
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Abstract: | The light chain composition of MS and SSPE CSF oligoclonal IgG bands was examined using isoelectric focusing and sensitive peroxidase-anteperoxidase (PAP) staining technique specific for gamma heavy chains (γ), kappa light chains (κ), or lambda light chains (λ) and a radioimmunoassay (RIA) for γ, κ, or λ. Many bands in the 7 MS and 4 SSPE CSF examined were monoclonal, staining for either IgG-κ or IgG-λ. By staining, all MS CSF were κ predominant; SSPE CSF were variously κ orλ predominant. RIA confirmed the κ predominance of MS CSF. Three MS and 2 SSPE CSF contained bands staining for λ alone, i.e. free light chains. Analysis of RIA data confirmed these findings in 2 MS cases. The difference in light chain predominance of MS and SSPE CSF may reflect differences in the antigenic target, or the age of patient at the time when band-synthesizing clones are triggered. Six of 7 MS and all 4 SSPE CSF contained oligoclonal bands staining for γ and for both κ and λ, probably representing artifacts of IEF. No predominant immunochemical differences between bands in MS and SSPE were detected. |
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Keywords: | Cerebrospinal fluid IgG bands Light chain composition Multiple sclerosis Radioimmunoassay Subacute sclerosing panencephalitis |
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