淫羊藿对运动训练大鼠睾酮含量、物质代谢及抗运动疲劳能力的影响

 目的 研究淫羊藿对运动训练大鼠睾酮含量、物质代谢及抗运动疲劳能力的影响。方法 以大强度耐力训练大鼠为模型,50只42 d龄雄性Wistar大鼠为对象,随机分为3组：静止组、运动组、淫羊藿组,每组15只（剔除不符合实验要求的大鼠）。采用专业灌胃器每天灌胃1次,淫羊藿组剂量为5 g·kg^-1,灌胃体积为5 mL·kg^-1,静止组、运动组灌胃等量生理盐水。42 d力竭游泳训练后,测定体重、力竭游泳时间及血睾酮等生化指标。结果 淫羊藿组体重大于运动组（P<0.05）,力竭游泳时间长于运动组（P<0.01）。力竭游泳导致大鼠血清睾酮下降,运动组与静止组比较,下降了28.85%(P<0.01)。淫羊藿组与运动组比较,上升了28.21%(P<0.01);血清皮质酮水平各组间均无显著差异,血清睾酮与皮质酮比值变化与睾酮变化较为一致;肝糖原、肌糖原储量下降,运动组与静止组比较,肝糖原下降26.40%(P<0.01),肌糖原下降54.83%(P<0.01)。淫羊藿组与运动组相比,肝糖原高出20.68%(P<0.05),肌糖原高出82.76%(P<0.01);促卵泡激素无明显影响;血尿素上升,血红蛋白下降,运动组与静止组比较,血尿素上升了66.32%(P<0.01),血红蛋白下降35.89%(P<0.01)。淫羊藿组与运动组相比,血尿素低出27.85%(P<0.01),血红蛋白高出41.23%(P<0.01)。结论 淫羊藿可以减轻血睾酮受高强度运动量的影响,并维持在正常生理水平;可以促进蛋白质合成,抑制氨基酸和蛋白质分解,提高运动训练大鼠血红蛋白含量和糖原的储备。

Effect of Epimedium davidii on Testosterone Content,Substance Metabolism and Exercise Capacity in Rats Receiving Exercise Training

OBJECTIVE To study the effect of Epimedium davidii on the content of testosterone,substance metabolism and anti-fatigue ability of rats. METHODS High-intensity endurance training rat model was used. Fifty 6-week-old male Wistar rats were divided into 3 groups,with 15 in each group(the rats which did not meet the requirement were removed) still ig water group (C group),exercise ig water group(T group),and exercise ig Epimedium davidii group(TM group). Gavage was performed using professional device once a day. The rats in TM group were administered at a dose of 5 g·kg^-1 with ig volume of 5 mL·kg^-1. The rats in C and T groups were given saline of same volume. After 42 d of exhaustive swimming training,body weight,swimming time and serum testosterone and other biochemical markers were measured. RESULTS The body weights of the rats in TM group were greater than those in T group(P<0.05),and the swimming time was longer than T group(P<0.01). Exhaustive swimming led to decreased serum testosterone,for which T group decreased by 28.85% more than C group(P<0.01). Compared with T group,TM group increased by 28.21%(P<0.01)in serum testosterone; the serum corticosterone levels in each group showed no significant differences,and the serum testosterone/corticosterone ratios changed in line with testosterone; liver glycogen and muscle glycogen reserves decreased. Compared with C group,the T group's glycogen decreased by 26.40%(P<0.01),muscle glycogen decreased by 54.83%(P<0.01). Compared with T group,TM group had 20.68% higher liver glycogen(P<0.05)and 82.76% higher muscle glycogen(P<0.01); FSH had no significant difference; blood urea increased and hemoglobin decreased compared with C group. Blood urea of T group increased by 66.32%(P<0.01),and the hemoglobin decreased by 35.89%(P<0.01); compared with T group,TM group had 27.85% lower blood urea(P<0.01)and 41.23% higher hemoglobin(P<0.01). CONCLUSION Epimedium davidii can alleviate the impact of high-intensity exercise on serum testosterone,and maintain it at normal physiological level; it can also promote protein synthesis,inhibit degradation of amino acid and protein,and increase hemoglobin and glycogen reserves in rats receiving exercise training.