传染性非典型肺炎病例SARS冠状病毒特异性抗体检测及分析

目的　对福建省传染性非典型肺炎病例血清标本中SARS冠状病毒 (SARS -CoV)特异性抗体进行检测。方法　采用间接酶联免疫吸附试验 (ELISA)测定SARS临床诊断、疑似、医学观察病例以及其他非SARS患者和健康人群血清SARS -CoV特异性IgM、IgG抗体。 结果　 (1) 3例SARS临床诊断病例血清SARS -CoV特异性IgG抗体均为阳性 ,其中 2例IgM抗体阳性 ,2例恢复期血清比急性期血清IgG抗体呈≥ 4倍增长 ;(2 ) 1例疑似病例IgM和IgG抗体均为阳性 ,且恢复期血清比急性期血清IgG抗体呈 >4倍增长 ;(3) 11例医学观察病例IgM和IgG抗体均为阴性 ;(4 ) 5 0例非SARS患者和健康人群IgG抗体全部阴性。 结论　间接ELISA是传染性非典型肺炎实验室特异性检测方法 ,可对SARS临床诊断进行进一步的核实。

Detection and analysis of the specific antibodies against SARS coronavirus in patients with SARS

Aim To detect the specific antibodies against the SARS coronavirus (SARS-CoV) in the sera of patients with SARS.Method IgM and IgG antibodies against the SARS-CoV in the sera of clinically diagnosed,suspected and medically observed cases and other non-SARS and healthy contrast were detected by the indirect enzyme linked immunosorbent assay.Results (1) All 3 clinically diagnosed cases were IgG positive,2 were IgM positive,2 paired sera (acute stage and convalescent stage) showed ≥4 fold rises in IgG.(2) 1 suspected case were positive for both IgM and IgG,and the paired sera showed >4 fold rises in IgG.(3) 11 medically observed cases were negative for both IgM and IgG.(4) None of the 50 samples from non-SARS and healthy contrast samples showed IgG positive.Conclusion Indirect enzyme linked immunosorbent assay can be used for the laboratory diagnosis of SARS and further clinic diagnosis checking of SARS.