前胡香豆素类提取物的UPLC/Q-TOF-MS分析及其初步药效学研究

目的建立UPLC/Q-TOF-MS法分析前胡香豆素类提取物的化学成分,并对其初步药效学进行研究。方法采用Acquity UPLC BEH C_(18)色谱柱;以0.1%甲酸水溶液(A)-乙腈(B)为流动相,梯度洗脱;检测波长321 nm;采用电喷雾正离子化模式(ESI~+),扫描范围m/z 100~1 000;利用链脲佐菌素(STZ)诱导的2型糖尿病大鼠模型,对前胡香豆素类提取物的调脂降糖作用进行初步药效学研究。结果前胡香豆素类提取物中的qianhucoumarinD(1)、peucedanocoumarinⅡ(2)、白花前胡甲素(3)、peucedanocoumarin I(4)、白花前胡乙素(5)、praeuptorin E(6)得到良好的分离与鉴定。初步药效学实验结果表明,前胡香豆素类提取物能显著降低2型糖尿病大鼠空腹血糖、胆固醇、三酰甘油、低密度脂蛋白及肝脏指数(P0.05),升高高密度脂蛋白(P0.05),提示前胡香豆素类提取物能改善2型糖尿病大鼠糖与脂质代谢,发挥治疗2型糖尿病的作用。结论建立了前胡香豆素类的UPLC/Q-TOF-MS的分离鉴定方法;初步药效学研究表明前胡香豆素类提取物具有显著的调脂及降糖作用。

Analysis on Peucedani Radix coumarin by UPLC/Q-TOF MS and study on its preliminary pharmacodynamics

Objective To establish a UPLC/Q-TOF MS method for analyszing the chemical constituents of total coumarins extract from Peucedani Radix (the rhizome of Peucedanum praeruptorum) and to study its preliminary pharmacodynamics. Methods The chromatographic column was Acquity UPLC BEH C₁₈, the mobile phase was composed of 0.1% formic acid (A) and acetonitrile (B) with gradient elution; The detection wavelength was set at 321 nm; The ionization mode was ESI⁺; And the mass scan range was m/z 100-1 000. Using the rat model of type 2 diabetes mellitus (T2DM) induced by streptozocin (STZ), the effect of total coumarins on reducing blood-lipid and blood-sugar was investigated in the experiment. Results Qianhucoumarin D, peucedanocoumarin II, praeruptorin A, peucedanocoumarin I, praeruptorin B, and praeuptorin E in Peucedani Radix coumarin could be well separated and identified in one run. The preliminary pharmacodynamic results show that the total coumarins had an effect of reducing the level of fasting blood glucose, cholesterol, triglycerides, LDL, and liver index (P < 0.05); On the other hand, it could improve the level of HDL (P < 0.05). The above results prompt that coumarin extract of Peucedani Radix could improve the carbohydrate and lipid metabolism of T2DM, then play a role in the treatment of T2DM. Conclusion A UPLC/Q-TOF MS method has been established to separate and identify the total coumarin extract of Peucedani Radix, the preliminary pharmacodynamic study shows that the total coumarin extract of Peucedani Radix had the function of reducing blood sugar and blood lipid significantly.