聚醚醚酮表面多孔羟基化改性对MC3T3-E1细胞黏附、增殖的影响

目的对聚醚醚酮(polyetheretherketone,PEEK)薄片表面进行多孔化和羟基化改性,观察PEEK表面形貌和生物活性的变化,并探讨该改性方法对前成骨MC3T3-E1细胞黏附、增殖的影响。方法超声波环境下浓硫酸处理PEEK表面,在其表面形成大量微孔结构;经湿化学法将PEEK表面的酮类基团还原成羟基基团,改善其表面化学活性,提升PEEK薄片的生物相容性。利用扫描电子显微镜(SEM)、傅里叶变换红外光谱仪(FTIR)及静态水接触角检测改性前后材料表面形貌、化学基团及亲水性的变化。未处理PEEK、多孔化PEEK、羟基化PEEK、多孔羟基化PEEK与MC3T3-E1细胞共培养,评价表面改性后PEEK薄片对细胞黏附、增殖的影响。结果 SEM结果显示浓硫酸处理后的PEEK薄片表面形成密集的空隙大小均匀的微孔结构,FT-IR结果证实羟基化改性成功地在PEEK表面还原出了大量羟基基团。同时,表面多孔化和羟基化改性均可有效提升PEEK材料表面的亲水性能。在体外细胞实验中,不同改性的PEEK材料与MC3T3-E1细胞共培养后结果显示,多孔化、羟基化和多孔羟基化改性均可显著促进细胞黏附和伸展,同时随着时间的延长,其促进细胞增殖的功能也逐步增强。结论表面多孔羟基化改性能有效提高PEEK材料表面的生物学活性和亲水性能,进而显著促进细胞的黏附和增殖。

Porous and hydroxylated modification on the surface of PEEK and its effect on the adhesion and proliferation of MC3T3-E1 cells

Objective To observe the surface morphology and bioactivity and to investigate the effect on the adhesion and proliferation of MC3T3-E1 cells after the porous and hydroxylated modification on the surface of polyetheretherketone(PEEK). Methods In order to improve the surface chemical activity and promote the biocompatibility of PEEK, the porous structure on the surface of PEEK was prepared by immersing into concentrated sulfuric acid under ultrasonic condition and the hydroxyl groups on the surface of PEEK was achieved from the reduction reaction of the carbonyl groups using wet chemical methods. The changes of morphology, chemical groups and hydrophilicity on the surface of PEEK were characterized by scanning electron microscopy (SEM), fourier transform infrared spectroscopy (FT-IR) and static water contact angle measurement before and after the PEEK modification. Untreated PEEK, porous PEEK, hydroxylated PEEK and porous-hydroxylated PEEK were co-cultured with MC3T3-E1 cells to evaluate the effect on the cells adhesion and proliferation after the surface modification of PEEK. Results SEM showed that dense homogeneous porous structure was observed on the surface of PEEK modified by concentrated sulfuric acid. FT-IR demonstrated that massive hydroxyl groups were successfully reduced on the surface of hydroxylated PEEK. Furthermore, the surface porous and hydroxylated modifications could effectively improve hydrophilicity of PEEK. In vitro, the results of MC3T3-E1 cells after co-culturing with different modified PEEK materials demonstrated that cells adhesion and spreading greatly improved and the capacity of cells proliferation gradually enhanced over time after the surface porous, hydroxylated and porous-hydroxylated modification. Conclusion The surface porous-hydroxylated modification can effectively improve the surface bioactivity and hydrophilicity of PEEK, and then further promote cells adhesion and proliferation