HSPCO16重组蛋白对毛乳头细胞增殖的影响

目的观察HSPCO16重组蛋白对培养的低/高传代毛乳头细胞(DPC)的影响。方法消化对数期生长的第2代和第7代DPC,调整细胞浓度为3×105细胞/mL,接种于24孔细胞培养板;至对数生长期时加入HSPCO16重组蛋白,调整浓度为0.1mg/mL继续培养,倒置显微镜下定期观察;一组细胞采用流式细胞仪进行检测;另一组细胞内加入3H-TdR继续孵育6h,消化收集细胞,检测HSPCO16重组蛋白对DPC增殖的影响。结果HSPCO16重组蛋白处理的DPC增殖活跃,生长状态明显好于对照组;流式细胞仪分析和3H-TdR掺入试验均证实重组HSPCO16蛋白可促进第8代DPC的增殖及其DNA合成,而对第3代DPC则无明显作用。结论HSPCO16重组蛋白具有促进高传代DPC增殖的作用,可能是一种新的促进毛囊生长蛋白。

Effects of The Recombinant HSPCO16 Protein on Proliferation of Dermal Papilla Cells in vitro

ObjectiveTo observe the effect of the recombinant HSPCO16 protein on low-/high passage dermal papilla cells(DPC).MethodsTo digest the passage 2 and 7 DPCs in log growth phase and to adjust the cell concentration to 3×105 cells/mL and incubate the DPCs to 24 hole cell culture flask.The recombinant HSPCO16 protein was added to DPCs in log growth phase and the concentration of protein was adjusted to 0.1mg/mL.The cells were observed by phase-contrast light electron microscopy.One group of cells was measured by flow cytometer,and the other group was supplemented with 3H-TdR incorporation and incubated for 6 hours.The cells were digested and the proliferative capacity was measured.ResultsThe DPCs treated with recombinant HSPCO16 protein proliferated more actively than that of control DPCs.The results of flow cytometer and 3H-TdR incorporation showed that recombinant HSPCO16 protein promoted the proliferation and DNA synthesis of passage 8 DPCs.However,HSPCO16 protein had no effect on passage 3 DPCs.ConclusionRecombinant HSPCO16 protein had the effects of promoting the proliferation of high-passage DPCs and maybe a new protein to up-regulate the hair follicular growth.