叶黄素对牛晶状体上皮细胞增殖及迁移的影响

目的观察叶黄素（lutein）对体外培养的牛晶状体上皮细胞（bovine lens epithelial ceHs，BLECs）增殖和移行的影响．为药物防治后发性白内障（after-cataract）提供新的选择。方法首先进行BLECs原代培养和传代培养。取第2～3代BLECs。分别采用MTT法、划线法．研究不同浓度及作用时间的叶黄素对体外培养的BLECs增殖及移行的影响。结果①与对照组相比．当浓度〉μmol／L时，叶黄素能够明显抑制BLECs的增殖．差异有显著性（P〈0．01）。相同作用时间不同浓度组之间抑制作用比较，随药物浓度的增加，抑制作用加强，各浓度组之间差异有显著性（P〈0．01）。而相同药物浓度组随着作用时间的延长，抑制作用加强，各时间点差异亦有显著性（P〈0．01）。②浓度为1μmol和2μmol／L的叶黄素能明显抑制BLECs的的移行，与对照组相比，差异有显著性（P〈0．01）。两组间愈合率比较，差异亦有显著性（P〈0．01）。结论①在浓度≥1μmol时，叶黄素能有效抑制BLECs的增殖。②浓度为1μmol／L和2μmol的叶黄素能抑制BLECs的移行，其强度呈一定时间依赖性。③叶黄素可能成为药物治疗后发性白内障的新选择。

Effects of lutein on the growth and migration of bovine lens epithelial cells in vitro

Objective To observe the effects of lutein on the growth and migration of bovine lens epithelial cells （BLECs） in vitro and to find a drug that can be used after cataract surgery. Methotis In this study, BLECs were cultured in vitro first and then different concentrations of lutein were added to the epithelial cell cultures of the second and third generations. The effects of lutein on the proliferation of BLECs in vitro were examined by the MTT method, and the migration of BLECs was evaluated by a wound scratch assay. Results （1）With lutein treatment varying from concentrations of 1μmol/L to 16 μmol/L, the proliferation of BLECs decreased in a dose-and time-dependent manner （P〈0.01）. （2）The migration of BLECs was evaluated by wound healing rate. When compared to the control group, the wound healing rate of the experimental groups decreased from 0.672±0.164 to -0.234±0.144 and -0.597±0.063 （P〈0.01） at 1 pμmol/L and 2 pμmol/L, respectively. Conclusion （1）Specific concentrations of lutein inhibited the proliferation as well as the migration of BLECs in vitro. （2）Lutein may become an effective drug to use after cataract surgery.