一氧化氮在阻塞性黄疸病人肝脏损害中的作用

目的　探讨一氧化氮 (NO)在阻塞性黄疸病人肝脏损害中的作用。方法　选取 30例阻塞性黄疸 (OJ)病人和 30例对照 ,采用免疫组化方法检测肝脏组织诱生型一氧化氮合酶 (iNOS)的表达 ,用硝酸还原酶法测定外周血和胆汁中一氧化氮水平 ,用放射免疫法检测肿瘤坏死因子 (TNF) ,计算机图像分析仪对肝脏组织坏死面积进行检测。结果　 (1)OJ组外周血NO、GPT、TBil、TNF和胆汁中NO水平较对照组显著增高 (P <0 0 1) ;(2 )iNOS在 86 7%的OJ肝脏组织中阳性表达 ,在对照组肝脏组织中不表达 ;(3)肝脏组织坏死面积与iNOS表达强度及NO水平呈显著正相关 (P <0 0 1)。结论　NO参与了阻塞性黄疸病人肝脏损害的过程并起重要作用。

Role of nitric oxide in hepatic injury of patients with obstructive jaundice

Objective To investigate the role of nitric oxide (NO) in hepatic injury of patients with obstructive jaundice (OJ). Methods Thirty patients with OJ were selected as the experimental group and 30 patients with cholelithiasis were selected as the control group. Serum and bile nitrite/nitrate levels were measured as indexes for NO generation in the patients. Tissue sections from 30 patients with OJ were immunohistochemically examined for protein expression of iNOS and hepatic tissue necrosis area (HTNA) was calculated by image analysis system using HE staining. Results 1) The levels of NO, GPT, GOT, TBil were significantly higher in the experimental group than in the control (P<0 01). 2) Positive iNOS immunostaining was detected in 86 7% of the patients with OJ but not in the control group. 3) HTNA was positively correlated to intensity of iNOS expression and NO level (P<0 01). Conclusions NO plays an important role in hepatic injury of patients with OJ.